In not more than five lines, outline a method for preparation of tomato cell suspension culture and subculture.
1. Callus: Plant callus in simple terms is a growing mass of unorganized parenchyma plant cells that cover a plant wound.
2. Cell suspension culture : Cell Suspension culture is different form of culture in which single cells or very small aggregates of cells multiply while they are suspended in agitated liquid medium for specific amount of time.
3. Subculture : A subculture is defined by transferring some or all cells from a previous old saturated culture to fresh growth medium and hence used to prolong the life and/or expand the number of cells or microorganisms in the culture.
4.Media:The best regeneration medium is the Murashige and Skoog (MS) medium for tomato cell growth.
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