Define a pure culture.

Q: What is the difference of washing from hand disinfection?

A: Introduction:- Hand hygiene is very important for health. Hands are very important route of germs…

Q: Illustrate the step by step method of preparing culture media.

A: Introduction Culture medium:- It is a liquid or gel designed for specific mixtures of nutrients and…

Q: Find the amount of dehydrated medium needed to prepare 15 BUTTS, 20 SLANTS, and 25 BUTT-SLANTS.

A: Slants, Butt-slants, and butts are different types of media prepared for bacterial growth. Slants…

Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…

A: Generation time is the time which is taken by the bacteria to double in number during a specified…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: Why is the culture medium referred to as a highly enriched laboratory soil?

A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…

Q: Write an introduction related to culture media and isolation of pure culture technique (streak plate…

A: In microbiology, there are five I’s which represent five different techniques used in the…

Q: aseptic technique and pure culture technique

A: A‌SEPTIC TECHNIQUE_ *Inserting PICC lines. *Performing dialysis.‌ *Inserting catheters.‌ *Running…

Q: Discuss the most important thing to remember in preparing and inoculating culture media?

A: Introduction: Microbes are a source of contamination and infection. They are categorized as…

Q: _____ kills pathogens and some spoilage organisms but does not sterilize substances. Tyndallization…

A: Sterilization refers to any process that removes, kills, or deactivates all forms of life, in…

Q: List the two main types of culture media and how each differs and the types of microbes which grow…

A: Culture media contains all the elements which the bacteria needs for growth and is not selective in…

Q: What is a pure culture?

A: Introduction  Culture is referred to th process of growing the cell outside the animal or plant body…

Q: Does the image above represent a pure culture? Yes O No

A: Answer- No

Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…

A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…

Q: Describe ways in which anaerobes may be cultured.

A: Answer: Introduction: An anaerobic organism or anaerobes are any type of organism that can grow…

Q: Differentiate moist heat from dry sterilizing heat.

A: Dry heat sterilization:-one of the oldest techniques of sterilisation is dry heat sterilisation of…

Q: Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes…

A: * Disinfectant is a chemical substance that inactivates or to destroy the microorganisms that are…

Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…

A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…

Q: What should be taken into consideration in the selection of the culture preservation method?

A: The problem of maintaining microbial culture is one that many fields of microbiology face. The…

Q: Identify applications for ethylene oxide sterilization.

A: KEY WORDS :- Ethylene oxide - It is a gas which is flammable in nature and colorless which is used…

Q: yield

A: The Dische's test is a chemical test that is utilized to detect the presence of DNA in a solution.It…

Q: What temperature is considered adequate for achieving sterilization of a compost pile?

A: temperature considered for achieving sterilization of a compost pile is 135-160 F. At a…

Q: Enumerate and describe briefly the components of a culture medium and indicate their  uses

A: Culture medium is a preparation for the growth or cultivation of microbes in vitro and includes…

Q: Describe the step by step method of preparing culture media in microbiology.

A: Culture media are the media or the substrate used to grow microorganisms.  There are different types…

Q: Describe and give the principle of each equipment used for sterilization in microbiology.

A: Sterilization is the method of removal, killing or inhibition of all forms of lives, spores or…

Q: Provide three reasons why the use of aseptic technique is essential when handling microbial cultures…

A: Aseptic techniques means using practices and procedures to prevent contamination from pathogens. It…

Q: Give at least two other examples of samples that are best prepared using Smear Preparation…

A: Smear preparation technique: it is used to fix the samples onto the slides.

Q: List five essential procedures of the aseptic technique when transferring a broth culture to another…

A: The term "aseptic method" refers to a combination of medical practises and procedures that help…

Q: chemically defined media  b.    complex culture media

A: Media is a growth medium of solid,liquid and semi solid type. Diffarent types of media are used for…

Q: Describe the five phases of a microbial growth curve observed when microbes are grown in a batch…

A: Introduction: Bacteria is unicellular prokaryotic organism that can survive in any kind of…

Q: What is meant by the term “enrichment culture”?

A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…

Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…

A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…

Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…

A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…

Q: Differentiate between defined and complex culture media

A: To differentiate: Chemically defined and complex culture media

Q: Explain with the examples of biochemical test that are used to identify the bacteria in the…

A: The smallest organism that is usually defined that the are been can be seen with the naked eye is…

Q: list the reagents used in the IMViC tests and what they detect

A: IMViC is a series of tests that include the indole test, methyl red test, Voges-Proskauer test, and…

Q: Write down the materials, reagents, Principle, procedure  and results for Ziehl-Neelsen stain

A: It is a differential staining technique which was first developed by Ziehl and later on modified by…

Q: which kind of sample gives positive result for Molish test?

A: It is a chemical test which is named after the botanist Hans Molisch.  This test involves the…

Q: Describe the difference between batch and continuous culture techniques

A: Bacteria and fungi, for example, are extremely valuable to a wide range of industries.…

Q: Tell about enrichment culture techniques.

A: A microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: Illustrate the different solid tubed media formed  1. Deep/Butt solid tubed media 2. Slant solid…

A: The selection of media is a crucial point in determining the generation of contamination-free…

Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: escribe the examples of biochemical test that are used to identify the bacteria in the laboratory.

A: According to the definition of a biochemical test, it is a test that is used to distinguish between…

Q: What are the different types of Pasteurization?

A: Pasteurization is the process of heat processing the liquid (mainly milk) or food so that the…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.