attern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated.

Q: Illustrate the step by step method of preparing culture media.

A: Introduction Culture medium:- It is a liquid or gel designed for specific mixtures of nutrients and…

Q: A subculture is a a. colony growing beneath the media surface b. culture made from a contaminant c.…

A: We make subculture from a plate where many different colonies of bacteria are growing.

Q: the sabouraud and nutrient agar plates

A: Sabouraud agar or Sabouraud dextrose agar (SDA) is a type of agar growth medium containing peptones.…

Q: Explain whether the MacConkey agar plate inoculated with good water would show the presence of…

A: Water contamination is the presence of unwelcome particles in the water. Water bodies may become…

Q: A fastidious organism must be grown on what type of medium?a. general-purpose mediumb. differential…

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: Why is the culture medium referred to as a highly enriched laboratory soil?

A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…

Q: Some exconjugants did not grow on minimal medium. On what medium would they grow?

A: Exconjugants can be described as bacterial cells, which separates following the conjugation. A…

Q: The culture medium can be reffered to as a 'highly enriched labotary soil.justify this statement?

A: Culture medium:   It is also known as a growth medium where specific microorganisms and plants will…

Q: Define a pure culture.

A: A microbiological culture, or microbial culture, is a strategy for duplicating microbial life forms…

Q: Bacteria are not used as probiotic agents. True O False The growth of bacteria that form endospores…

A: Bacteria are prokaryotic microorganisms. Bacteria are ubiquitous in nature and are found almost in…

Q: How do you sterilize the following? petri dish inoculating loop culture media test tube…

A: Sterilization of following- petri dish - By using autoclave or by a series of steps that includes…

Q: The factors for the manipulation of culture medium?

A: Culture medium It is also called a growth medium. It is a solid, liquid, or semi-solid medium that…

Q: Motility is best observed with a a. hanging drop preparation c. streak plate b. negative stain d.…

A: Motility is the ability of bacterial cells to migrate from one location to another. The flagella…

Q: A fastidious organism must be grown on what type of medium?a. general-purpose mediumb. differential…

A: Bacteria require appropriate environmental conditions for their growth. Solid or a liquid…

Q: Describe (at the cellular level) how detergents like roccal and SDS (from solution 2 in the plasmid…

A: Plasmid extraction is the molecular technique to isolate plasmid DNA from a bacteria. This process…

Q: Citrate test What is the color of the medium before inoculation? Green or blue

A: Biochemical method of bacterial identification is a classical method that is used for the…

Q: why is it important that a small amount of inoculum be used in preparation of an agar slant

A: Agar is a jellylike substance derived from the purified cell walls of red algae. It is added to…

Q: Which of the following medium is considered an enriched medium? a. LB b. T-soy  c. Nutrient  d.…

A: A growth or culture medium is solid, liquid or semi solid in nature that is used to support the…

Q: What would you expect to see if you cultured Gram-positive, lactose-fermenting bacteria on MacConkey…

A: Given: What would happen if you culture gram positive, lactose fermenting bacteria in MacConkey…

Q: Show a concept map about the aseptic technique on Transfer from Broth to Broth Culture, Transfer…

A: Aseptic techniques or sterile techniques are procedures that ensure proper handling and conduction…

Q: Selective media: O Is regular nutrient agar that can grow any microorganism Selects for the growth…

A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…

Q: If one is trying to determine if a culture is pure or mixed, which medium would you choose? Why?…

A: In microbiology, to study the different types of microorganism we use different mediums which are…

Q: Media that prohibits the growth of some types of bacteria, but has no reaction to bacterial growth…

A: Introduction Microbiological media, often known as bacterial culture media, is a bacterial growth…

Q: The difference in the colony appearance of bacteria cells growing on mannitol salt agar is detected…

A: Mannitol salt agar is a selective culture media that is used for the isolation of the pathogenic…

Q: if straph are only suppose to grow on the plate what could possibly be wrong with the media ?

A: Staphylococcus aureus is a Gram-positive bacterium which is cocci shaped. It is a facultative…

Q: C MSA (Mannitol Salt Agar) Match the description to the correct Bacterium A= growth, agar is yellow…

A: Microbiology is the branch of science that deals with the study of microorganisms that are too small…

Q: To prepare nutrient agar medium, you have to pour approximately. mL of medium into the Petri plat a.…

A: A patri dish is a normal in which is used for the growth of bacteria and other microorganisms. it is…

Q: Why is a Bunsen burner used in the microbiology lab? (select all that apply) The flame is used to…

A: Answer -The flame is used to sterilize the inoculating loop. -The flame kills microorganisms that…

Q: Use of streak plate technique to isolate and purify psychrotrophic spoilage-causing bacteria

A: There are billions of bacteria in the human body that make up the natural flora to resist the…

Q: From the growth OD data alplot a growth curve graph with standard deviation bars blcalculate the…

A: Abstract Error bars commonly appear in figures in publications, but experimental biologists are…

Q: Which of the following medium would be most appropriate for the bacterial cultures from stomach? a.…

A: Microorganism culture out of their natural environment in pure form in the laboratory has been…

Q: :In bacterial culture media, agar is used as An additional nutrient .a Buffer .b A solidifying agent…

A: Agar is used in laboratory to help, feed and grow microorganisms. It is used in laboratory for…

Q: ow can one be sure that the culture media are sterile or free from contamination

A: Sterility, often known as sterility, is the state of being free of all contaminants and…

Q: Name the source from where agar is commercially obtained.

A: Agar is a jelly-like substance that is made up of linear polysaccharide agarose and a heterogenous…

Q: Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking.…

A: Streaking method This method is used to produce isolated colonies of bacteria. This helps in…

Q: Differentiate between defined and complex culture media

A: To differentiate: Chemically defined and complex culture media

Q: Colony formation can be observed in liquid media.  a) Trueb) False

A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…

Q: Agar is added to media.

A: Agar is obtained from algae. It is composed of agarose (polysaccharide) and agaropectin. It is a…

Q: Bacteria Reagent Gram-positive Gram- Primary dye Mordant Decolorization Şeçondary dye

A: Staining is a technique utilized in research to boost distinction within the microscopic image.…

Q: a. STREAK PLATE METHOD: a. Pour melted NA or CWA, following aseptic technique in a petri dish and…

A: The reason for the streak plate is to acquire separated colonies from an inoculum by making areas of…

Q: In a tabulated form, differentiate the physical and chemical methods of sterilization.

A: The microbiological technique that is performed for the removal, killing, or deactivation of…

Q: MSA is a selective medium and contains a higher salt concentration, a pH indicator and Mannitol…

A: Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and…

Q: The type of Isolation media that prevent the growth of unwanted bacterla without inhibiting growth…

A: A suitable nutrient medium is responsible for providing an appropriate biochemical environment for…

Q: Assume that you had plated your +PGLO culture on an LB agar plate. What result (number of colonies)…

A: +pGLO culture is a type of bacteria that has been genetically modified to produce a fluorescent…

Q: Elaborate on the advantages of examining colony in an agar plate.

A: An agar plate is a Petri dish which have a development medium set with agar, utilised to culture…

Q: Define the meaning of Surface sterilization of explants Surface sterilization of explants

A: Plant tissue culture is very helpful technique in which plants are grown in in vitro condition and…

Q: You are given a culture medium on which only Gram negative bacteria will grow. Organisms that are…

A: The medium in which only gram negative bacteria will grow prevents gram positive bacteria to grow in…

Q: main goal of motility agar

A: MICROBIAL GROWTH MEDIA- It is the culture media that serves as a nutrient media for the growth of a…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.