Please help! I think the answers I put are correct, just looking for some verification/explanation.. What is an example of a gain-of-function experiment?a) use of morpholinos to test the function of MyoDb) use of gene-targeting to test the function of MyoDc) turning 10T1/2 cells into myoblasts cells by transfection of MyoDd) all of the aboveQUESTION 32For her thesis project, a graduate student discovered a new gene (creatively called Muscle-1) and investigated Muscle-1's functions in muscledevelopment using knockout methodologies. Her preliminary studies showed that differentiated skeletal muscle is absent at all stages examined, butmyoblasts are present in normal numbers. What is a reasonable conclusion from these findings?a) Muscle-1 plays a role in both muscle determination and differentiationb) Muscle-1 plays a role in muscle determinationc) Muscle-1 plays a role in somite formationo d) Muscle-1 plays a role in muscle differentiationQUESTION 33Mice that are mutant for either MyoD or Myf-5 exhibit normal muscle formation at birth, whereas mice with mutations in both genes are completely devoidof skeletal muscle. What conclusion can be drawn from these results?a) MyoD and Myf5 are expressed in the same population of muscle precursor cellsb) MyoD and Myf5 have identical biochemical functions° c) MyoD and Myf5 exhibit genetic redundancy, although the mechanism isn't clear from the data provided.d) MyoD and Myf5 are expressed in distinct populations of muscle precursor cellsQUESTION 34A researcher treated 10T1/2 cells with a new chromatin-modifying drug to test how this drug affected developmental potential. She concluded that thedrug converted 10T1/2 cells to determined cells that were able to differentiate into only one of three possible cell types. What data would support thisinterpretation?a) no differentiation was observed unless the medium was switched from growth medium to differentiation mediumb) clonal analysis of treated cells showed that each resulting colony contained all three cell typeso c) high density (non-clonal) cultures of treated 10T1/2 cells contained all three cell typesd) clonal analysis of treated cells showed that each resulting colony contained only one cell type

Gain of function means when the foreign DNA is inserted in anyone of the concerned cells, the gene…

nple of a gain-of-function experiment? pholinos to test the function of MyoD e-targeting to test the function of MyoD 1/2 cells into myoblasts cells by transfection of MyoD move roject, a graduate student discovered a new gene (creatively called Muscle-1) and investigated Muscle-1's functions in muscle sing knockout methodologies. Her preliminary studies showed that differentiated skeletal muscle is absent at all stages examined, but present in normal numbers. What is a reasonable conclusion from these findings? lays a role in both muscle determination and differentiation lays a role in muscle determination ays a role in somite formation ays a role in muscle differentiation utant for either MyoD or Myf-5 exhibit normal muscle formation at birth, whereas mice with mutations in both genes are completely devoid

nple of a gain-of-function experiment? pholinos to test the function of MyoD e-targeting to test the function of MyoD 1/2 cells into myoblasts cells by transfection of MyoD move roject, a graduate student discovered a new gene (creatively called Muscle-1) and investigated Muscle-1's functions in muscle sing knockout methodologies. Her preliminary studies showed that differentiated skeletal muscle is absent at all stages examined, but present in normal numbers. What is a reasonable conclusion from these findings? lays a role in both muscle determination and differentiation lays a role in muscle determination ays a role in somite formation ays a role in muscle differentiation utant for either MyoD or Myf-5 exhibit normal muscle formation at birth, whereas mice with mutations in both genes are completely devoid

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter8: Muscle Physiology

Section: Chapter Questions

Problem 2TAHL

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