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![3.
4.
Experiment 4
Page 2
CHEM 125L
The standard solutions are prepared according to Table 1.
Final volume is 25.0 ml for each standard.
The solvent is 0.1 M nitric acid.
Do not use water to bring the volume to 25.0 mL.
Plot the data of [FESCN2+] vs. Absorbance.
Find the equation of the line.
You will need this for Part B.
Table 1
Vol. of 0.20 M
Fe(NOs)s (mL)
Standard Solution
Absorbance at 447
Vol. of 0.001 M
NaSCN (mL)
[FESCN2*]
Blank
10.0
10.0
0.076
1.0
1.
10.0
2.0
0-172
2.
10.0
3.0
0.340
10.0
10.0
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The concentration of FeSCN + 2 at equilibrium is equal to the concentration of added NaSCN:
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- Preparing a standard curve. 1. determine the absorbance max of [FeSCN]2+ it is 448.1 2. determine the concentration of FeSCN2+ in the stock solution. M1V1=M2V2 stock solution: ~0.200 M iron (iii) nitrate in 1M nitric acid intial volume: 0.3mL final volume: 10.3mL volume: 10mL ~0.002M potassium thiocyanate initial volume: 0.3mL final volume: 10.3mL volume: 10mL not sure if this is needed stock solutions: ~0.200M iron (iii) nitrate in 1M nitric acid: 0.207M ~0.002M iron (iii) nitrate in 1M nitric acid: 0.00209M ~0.002M potassium thiocyanate: 0.00193MWhat is the role of specific visualizing agents in a Thin layer Chromatography? Please shortly answer at your own words. Answer should be to the point (4-5 lines).Internal standard (As , Cs ), analyte with known concentration (Ax , Cx ) In a chromatographic equipment, a solution containing 0.006727 X and 0.008331 M S give peak area of Ax= 2316 and As= 207. To analyze an unknown sample, 5.0mL of 0.008331M S was added to 5.0mL of X, and the mixture was diluted to 50.0mL. This mixture gave a chromatography spectrum with area Ax= 3206 and As= 200. Find Cx.
- A sample of pure CaCO3 (FW= 100.1 g/mol) weighing 0.350g was dissolved in concentrated HCI (MW= 36.45 g/mol) and diluted to 250mL in a volumetric flask. A 50.0mL aliquot required 35.00mL of an EDTA solution for titration. A 5.0-mL water sample to be analysed for its hardness was diluted to 250mL. A 10.00mL aliquot required 24.50mL of the EDTA solution to reach the EBT endpoint. 1. Calculate the molarity of the EDTA solution. 2. Calculate the water hardness in pm CaCO3.a. What mass of Ag2SO4 is formed when 34 mL of 0.300 M AgNO3 are mixed with 26 mL of 30.0 g/L Na2SO4? b. Potassium can be determined by flame emission spectrometry (flame photometry) using lithium internal standard. The following data were obtained for standard solutions of KCI and an unknown containing a constant, known amount of Lil as the internal standard. The intensity of a blank was 0.04. Calculate the concentration of K in the unknown. ck, ppm Intensity of K emission Intensity of Li emission 5.0 34.7 5.8 20.0 110.2 9.1 Unknown 57.3 6.8For Indirect Iodometric Analysis of Copper... ~0.0896g KIO3 necessary to consume 350mL of 0.1 M Na2S2O3, Na2S2O3 is stored in an amber glass bottle until ready for use. Primary Standard KIO3 has 2g of KI, 50mL of DI water, and 10 mL of 1.0M HCl is added then immediately titrated with Na2S2O3 until medium yellow or straw... then 5mL of starch indicator is added and titrated again until blue black color turns clear. Unknown CuO use 1.2G of Unknown, 20mL of HNO3 heated until sample dissolved, 25 mL of DI water added and boiled until clear light blue color, after cooling 1:1 NH3 added (~34.47 mL of NH4OH reagent) until permanent deep blue color amine complex, 2g of NH4HF2 added and swirled until dissolved, 3 g of KI is added then titrated immediately with Na2S2O3 until brown color of iodide is nearly gone (brown milk color), 2 g of KSCN and 3 mL of starch indicator is then added with titration continuing until disappearance of new blue black color. 1. Na2CO3 is often added to thiosulfate…
- For Indirect Iodometric Analysis of Copper... ~0.0896g KIO3 necessary to consume 350mL of 0.1 M Na2S2O3, Na2S2O3 is stored in an amber glass bottle until ready for use. Primary Standard KIO3 has 2g of KI, 50mL of DI water, and 10 mL of 1.0M HCl is added then immediately titrated with Na2S2O3 until medium yellow or straw... then 5mL of starch indicator is added and titrated again until blue black color turns clear. Unknown CuO use 1.2G of Unknown, 20mL of HNO3 heated until sample dissolved, 25 mL of DI water added and boiled until clear light blue color, after cooling 1:1 NH3 added (~34.47 mL of NH4OH reagent) until permanent deep blue color amine complex, 2g of NH4HF2 added and swirled until dissolved, 3 g of KI is added then titrated immediately with Na2S2O3 until brown color of iodide is nearly gone (brown milk color), 2 g of KSCN and 3 mL of starch indicator is then added with titration continuing until disappearance of new blue black color. 4. Why is the starch indicator solution…If pH (± 0.5 pH units) strips were used to determine the equivalence point at pH=8.5. What is the relative error (%) in pH at the equivalence point? I assumed it should be 0.5/8.5 * 100 = 5.88, rounded to 5.9 for sig figs. but I got the answer wrong. Please help and let me know how to solve this.Calculate the [FeSCN2+] using volumes of stock solutions. Presume that all the SCN– ions react. STANDARD SAMPLE Volume of Fe(NO3)3 (mL) Volume of SCN- (mL) Volume of H2O (mL) [FeSCN2+] Absorbance 1 2.5 2.0 20.5 0.1918 2 2.5 1.5 21.0 0.3239 3 2.5 1.0 21.5 0.4965 4 2.5 0.5 22.0 0.6209 Stock [Fe(NO3)3] = 0.200 M, Stock [SCN-] = 0.0020 M Any help would be greatly appreciated Please and thanks :)
- A third spectrophotometric method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal standard. A standard containing 1.75 ppb Pb2+ and 2.25 ppb Cu2+ yields a ratio of (SA/SIS)std of 2.37. A sample of blood is spiked with the same concentration of Cu2+, giving a signal ratio, (SA/SIS)samp, of 1.80. Determine the concentration of Pb2+ in the sample of blood.An analyst determines the Cl^- content of rain water with ion chromatography. The data for the calibration line in the range 5 to 50 milli equivalents per liter (mEq/L) is shown in the table below. Additionally, a blank sample was analysed 5 times and the following results obtained: 1.1, 0.5, 0.1, 0.9 and 1.2 ppm Ct Calculate the detection limit (DL) for the method with a 98.3% confidence level. Use ALL data and show equationsThe detecton limits reported for Al with ICP-AES and GF-AAS are 2 ppb and 5 picograms, respectivly. If we assume that a 20 microliter sample volume is used for the GF-AAS determination, which method has the lower detection limit? (1 picogram = 10-12 gram.)
