ou followed all the steps correctly with a kit you bought last week. However, NO purified DNA came out of the miniprep (More than one answer possible). a) The plasmid was lost during culture growth b) Your tears from doing the same experiment again and again contaminated the reagents, causing them not to work c) Depurination of the DNA led to loss of genetic material d) Incomplete lysis of the cells after adding lysis buffer
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
You followed all the steps correctly with a kit you bought last week. However, NO purified DNA came out of the miniprep (More than one answer possible).
Plasmids exist naturally in bacterial cells . In some cases bacteria need to pick up plasmid from the environment , as they readily lose them during the antibiotic resistance and during the process of conjugation , as one of the daughter cell may not receive plasmid .
Depurination involves the loss of purine bases from DNA . In this process N- glycosyl bound to deoxyribose is broken by hydrolysis , leaving the DNA sugar -phosphate chain intact , producing an absic site.
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