ou obtained the following raw data when setting up a Biuret standard curve:   BSA (mg/ml) Absorbancy 540nm

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter1: Introduction
Section: Chapter Questions
Problem 1.9QAP
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You obtained the following raw data when setting up a Biuret standard curve:

 

BSA (mg/ml) Absorbancy 540nm
0 0.158
1 0.210
2 0.260
3 0.305
4 0.360
5 0.410
6 0.455
7 0.510
8 0.530
9 0.550
10 0.554

 

After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.251 was obtained.

Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).

 

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