Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter1: Introduction
Section: Chapter Questions
Problem 1.9QAP
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Question
You obtained the following raw data when setting up a Biuret standard curve:
BSA (mg/ml) | Absorbancy 540nm |
0 | 0.158 |
1 | 0.210 |
2 | 0.260 |
3 | 0.305 |
4 | 0.360 |
5 | 0.410 |
6 | 0.455 |
7 | 0.510 |
8 | 0.530 |
9 | 0.550 |
10 | 0.554 |
After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.251 was obtained.
Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
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