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- Compare and contrast anti-miRNA oligonucleotides, locked nucleicacids (LNAs), and antagomirs, which may eventually be used to treat certain forms of cancer.which enzyme is used in the processing of miRNAs that are encoded in the genome but not in the processing of exogenously added siRNAs?What is the principal difference between the binding ofsiRNA and miRNA with mRNA?
- What is the difference between how miRNA stops gene expression and how siRNA does?Explain why incoming DNA recognized by a shortRNA molecule expressed from the CRISPR region cannotbe completely foreign to the cell.miRNAs target endogenous mRNAs in a sequence-specific manner. Explain, conceptually, how one might identify potential mRNA targets for a given miRNA if you only know the sequence of the miRNA and the sequence of all mRNAs in a cell or tissue of interest.
- While miRNA response elements (MREs) may be located anywhere within an mRNA, they are most often found outside the coding region in the 5 or 3 UTR. Explain why this is likely the case given that miRNAs often target more than one mRNA.Do you think it would be easier to mutate the GR receptor or treat someone with a miRNA to overcome the negative aspects of the stress response? Why?Some eukaryotic mRNAs contain an AU-rich element in the 3’ untranslated region. What would be the effect on gene regulation by a microRNA (miRNA) if the AU-rich element was mutated or deleted?
- Define about miRNA response elements (MREs) ?Which of the following is NOT true of miRNAs? A. Pri-miRNAs are processed by Drosha in the nucleus to form pre-miRNAs which are cleaved by dicer in the cytoplasm to generate a miRNA. B. miRNAs always lead to the degradation of their mRNA targets. C. miRNAs can be encoded within the vertebrate genome within introns and within intergenic regions. D. miRNAs can generally target more than one mRNAWhy is an NFR needed at the core promoter for transcription to occur?