You are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium botulinum in commercial sterilization. Calculate F value when sterilized at 131 °C in canning processing.
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You are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium botulinum in commercial sterilization. Calculate F value when sterilized at 131 °C in canning processing.
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- Oven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect: Rationale:Alternative name for Moist-heat sterilization Select one: Oven sterilization Autoclaving Ionizing radiationA microorganism has a D135 of 2 min. How many microorganisms will be left in the food sample after heat treatment at 135°C for 14 min if the initial microbial contamination is 109 cells/g product?
- Which of the following is false?a) A high-level disinfectant cannot be used as a sterilant.b) Critical items must be sterilized before use.c) Low numbers of endospores may remain on semicritical items.d) Standard sterilization procedures do not destroy prions.e) Quaternary ammonium compounds can be used to disinfect food preparation surfaces.Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.Many antibacterial hand soaps contain the chemical agent _____ , which belongs to the _____ antimicrobial category. a. sodium hypochlorite; halogen b. triclosan; phenolic c. sodium chlorite; halogen d. ethanol; alcohol
- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperThe optimum growth pH for a microorganism is usually: 4,5,7 or 8
- Given the following common laboratory materials, which of the three methods of heat sterilization do you think is the most appropriate and practical to use in sterilizing them ? Why do you think so ? a) empty flask b)L-rod (glass rod c)10 ml glass pipet d) wire needles e)antibiotic solutionWhat is the point of the pressure in the autoclave sterilization?which of the following is best to sterilize heat-liable (sensitive) solutions? a, pasteurization b. autoclave c. dry heat d. filration e. none of the above