Outline the Evans blue method for counting viable and dead cells

Q: How does the use of DAPI disciminate between red blood cells and white blood cells and describe the…

A: DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent dye.

Q: What are the causes of thrombophilia? Describe the laboratory tests that are used to confirm the…

A: A brief explanation is given below.

Q: Compare to necrosis and apoptotic cell deaths and indicate differences between those

A: Multicellular creatures' cells go through numerous growth and death cycles. Cellular death is…

Q: What would happen to the red blood cells of a patient who was treated with 10% saline?

A: Red blood cells are the most common erythrocytes or blood cells in the circulatory system. It helps…

Q: What is a Biomarker?

A: Biomarkers or biological markers are the measurements of processes taking place inside the cells.…

Q: What are the different methods of cell lysis? Discuss in detail.

A: Common ways to perform cell lysis include using pressure, temperature changes, ultrasonic…

Q: What is the total dilution of Tube #4? Express the answer in exponential format. b. You plated 1 mL…

A: It is common practice to do viable count on various environmental samples in microbiology and…

Q: Briefly explain the differences between F+, F−, Hfr, and F′ cells.

A: In bacteria, genes are transferred by conjugation process in which a donor cell transfers genetic…

Q: List examples of lysogenic conversion

A: Bacteria are single-celled living beings. The cell structure is simpler than that of various living…

Q: Briefly describe the steps of platelet adhesion and aggregation.

A: Blood is the circulatory fluid of the body. It is a buffer that bears the fluctuations in the pH of…

Q: Explain about The syringe-type sampler with the help of diagram.

A: The syringe-type sampler is a sample collecting equipment that is used to draw a blood sample from…

Q: - Explain another methods by which cytotoxicity is measured ?

A: Certain substances or mediator cells have the capacity to kill live cells. can be triggered by a…

Q: Explain the structure of protective system in detail with necessary diagram ?

A: The mechanism of protection involves placing a barrier between the pathogen and the susceptible part…

Q: Name the tests we used to test for marcomolecules.

A: The names of the tests used to detect macromolecules.

Q: describe the steps involved in primary cell dissociation protocol, assessing cell viability and sub…

A: Answer :- As we know that Cell viability examines give a readout of cell wellbeing through…

Q: Describe the protocol to generate competent cells through electroporation method.

A: Electrocompetent cells use the electroporation process to function. Electrical pulses created pores…

Q: How can chemotaxis be measured quantitatively?

A: The response to a chemical stimulus is more precisely known as the chemotaxis. It is widely…

Q: Identify the principles of the lypophilization process and list down the precautions and limitations…

A: Lyophilization is a messy freeze-drying process that eliminates water from a product after it has…

Q: . List the other possible mechanisms of cell damage. Is it possible to restore the number of blood…

A: Radiation sickness Radiation sickness is a result of being exposed to a high dose of radiation in a…

Q: e blank with the correct dilution for each tube in the series. tou 50 µl of patient serum 100 µl of…

A: Dilution is the technique of lowering the concentration of a solute in a solution by merely adding…

Q: Define any five Natural Products with MedicalApplications ?

A: Answer: Introduction: A natural product means a chemical substance formed by a living organism or…

Q: Give five (5) other examples of samples that are best prepared using Smear Preparation Technique…

A: Smear preparations involve spreading cells from a culture in a thin film over a small region of a…

Q: What is plasminogen activator?

A: The process of clotting blood in the body is a very complex process involving a multitude of…

Q: Why plasma cell are helpful ?

A: Blood is a connective tissue that contains cells and plasma. The cells that are present are red…

Q: What is the principle of counting with a hemocytometer? What are the advantages and disadvantages?

A: Answer: HEMOCYTOMETER : It is the tool used in most of the biochemistry and biology labs in order to…

Q: Which objectives might you be able to resolve mitochondria (approx. diameter 1um)? A red blood cell…

A: A light power microscope can be used to see objects as small as 0.2 micrometers (um). So, a light…

Q: Beginning with 2.0 ×104 cells/L, outline the Evans Blue method for counting viable and dead cells.

A: Viability assays are based on either the physical properties of viable cells, such as membrane…

Q: explain What do hemolysis and crenation

A: In short hemolysis is the destruction of RBC or red blood cells. Which means RBC swells up and burst…

Q: Illustrate the step-wise procedure of making a blood smear.

A: Phlebotomy refers to the process of puncturing the vein with a cannula to collect blood. Blood…

Q: Explain the structure of basophils?

A: Basophils are a type of WBCs (white blood cells). They are the least common type of the…

Q: methods of cell lysis?

A: Note: As per the guidelines, only one question has to be answered. Hence, 3.(a) will be answered as…

Q: Explain the principle involved in blood typing.

A: The most significant blood typing is based on ABO groups, though 20 different blood groups are known…

Q: chromatography and what are its effecting factor.

A: Chromatography is a very important biophysical technique that permits the separation,…

Q: Based on these blood agar results, can you identify what type of hemolysis are occurring in these…

A: Hemolysis is the destruction of red blood cells. It can occur due to different causes and leads to…

Q: L sample diluted 1:1 with trypan blue is counted using a hemocytometer. A total of 325 viable cells…

A: The hemocytometer is a counting chamber device that was originally created to count blood cells and…

Q: Describe the structure of Basophils?

A: Basophils are least abundant (0.5-1%) of the total WBCs. In these cells the nucleus is generally…

Q: Describe the hygiene hypothesis and the white blood cells involved.

A: white blood cells are blood cells which are part of the bodies defense against pathogens . the…

Q: Explain What are Nod factors and what do they do?

A: Nod factors (NFs) are basically lipochitooligosaccharide molecules discharged by rhizobia, which are…

Q: What is the principle of Immunochromatographic system, and its uses?

A: Immunology is the branch of science that deals with the study of immune systems and their…

Q: What is Cotransduction frequency?

A: Transduction is a process of genetic recombination in bacteria wherein a virus serves as a vector of…

Q: Provide information about the hemocytometer. Explain the principle of counting with hemocytometer…

A: The hemocytometer is a specimen slide which is used to determine the concentration of cells in a…

Q: Explain the principle and step by step procedure of Dry Blood Spots collection

A: Blood sampling Venous blood sample is usually preferred for routine laboratory test. Arterial blood…

Q: • Using specific examples, outline some methods to measure cell death by flow cytometry.

A: Cell death is a characteristic part of the lifecycle of a cell. In instances of advancement, it is…

Q: H1. Estimate the TKN associated with a sample having 50 mg/L of cell tissue and 10 mg/L of ammonia.…

A: TKN (Total Kjeldahl Nitrogen) test is a method of estimation of total concentration of organic…

Hematology

Hematology is the branch of biology. Hematology is the medical field that deals with the treatment of blood disorders and cancers, such as hemophilia, leukemia, lymphoma, and sickle-cell anemia. Hematologists and hematopathologists are medical professionals who specialize in illnesses of the blood and its components.