Why do you need to calculate for volume of media needed after computing the cells? If you are provided with a cell sample, how will you be able to find out its concentration by using a hemocytometer?
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TOPIC: CELL COUNTING METHOD (Molecular biology and
- Why do you need to calculate for volume of media needed after computing the cells?
- If you are provided with a cell sample, how will you be able to find out its concentration by using a hemocytometer?
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- EXPERIMENT : CELL COUNTING FOR DETERMINING VIABLE CELL CONCENTRATION Objectives: To count adherent and suspension cell using hemocytometer. To calculate viable cell concentration in a suspension. Procedures: Counting Viable Cells using Hemocytometer/Automated Cell Counter▪ Prepare the hemocytometer slide by cleaning the surface with 70% ethanol carefully (do not scratch the semi-silvered coating). Apply the same step for the coverslip. Wet the edges very lightly and press it down over the grooves and semi- silvered counting area. ▪ Collect 20 μl samples from the cell suspension using a pipettor and transfer it immediately to the edge of the hemocytometer chamber. ▪ Expel the suspension and allow it to be drawn under the coverslip by capillary force. Do not overfill the chamber. Blot off any surplus liquid and place the slide under the microscope. ▪ Alternatively, trypan blue dye can be used to stain cells by mixing an equal volume of trypan blue to a cell suspension (1:1) to…Despite widespread automation, why is it important for medical technologists to know the basics on the hemocytometer and how to use it?How does the microscopy in Figure 2 show that the capsule and an S-layer can exist in the same cell at the same time? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
- Please answer fast Can we use human blood for the preparation of blood agar? yes or No and explain why in full detail.Answer the below questions on “Absolute cell counts using a Neubauer haemocytometer ” 1)What is this test? 2)How is it carried out/what are the key aspects of the technique needed for accuracy? 3)What can it tell us? 4)What are the sources of error that could impact your data and interpretation?Please help me answer how much volume of cell suspension needed (for 5*10^4 cells) in microliters. Pertinent Information shown in the image.
- Please written by computer source 1. We want two T-75 flasks, each with 200,000 cells in the flask that holds 20 mL of culture medium per flask. Hemocytometer counting of cells in a 1 mm sq2 area gave an average of 12 cells from a 1:9 dilution of the cell suspension. a.) Determine the cells/mL. b.) What is the volume of your cell suspension needed to make the flasks? c.) How you would now make the flasks as a new subculture of cells for incubation?A Manton-Gaulin homogenizer is used for cell lysis. Which of the following are true? Increasing the number of passes can increase fine cell debris and make subsequent clarification more difficult. Cell growth conditions and phase of growth at which cells are processed can impact cell disruption. Decreasing temperature can increase protein release. Decreasing pressure reduces the number of passes required. Explain what is meant by the extent of cell disruption is 0.9.Write down a comparison of the sizes of the untreated cell and the colchicine-treated cell.
- State in details, what the key aspects of “Neubauer haemocytometer” technique that are needed for accuracy and what this technique can tell us.Match the following experimental methods with their specialty procedures and objectives. autoradiography use of green fluorescent protein (GFP) fractional centrifugation use of genetic mutants A. silencing genes means inhibition of the expression of a protein B. fluorescing GFP protein is fused with a protein tracked in the cell C. biochemical processes are visualized using radiolabeled materials D. tissue samples homogenized at varying centrifugal force leads to isolation of desired cellular fractionWhat is the principle of counting with a hemocytometer? What are the advantages and disadvantages?