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peptide Lys-Glu-Trp  answer the following questions:         Draw the structure of this peptide when all the ionizable groups are fully protonated.           How many ionizable groups does this peptide have? Label the pKa for each of the ionizable groups in the structure above.  Draw the appropriate titration curve for this peptide on graph paper starting at pH 0 and ending at pH 14. Label the x-axis, y-axis and the pKa  Determine the overall net charge of this peptide at each full equivalent point (including 0) where the ionizable group is 100% deprotonated.   Determine the pI for this peptide. Determine the average overall net charge of a mixture of this peptide in a solution where pH=8.5.

peptide Lys-Glu-Trp  answer the following questions:         Draw the structure of this peptide when all the ionizable groups are fully protonated.           How many ionizable groups does this peptide have? Label the pKa for each of the ionizable groups in the structure above.  Draw the appropriate titration curve for this peptide on graph paper starting at pH 0 and ending at pH 14. Label the x-axis, y-axis and the pKa  Determine the overall net charge of this peptide at each full equivalent point (including 0) where the ionizable group is 100% deprotonated.   Determine the pI for this peptide. Determine the average overall net charge of a mixture of this peptide in a solution where pH=8.5.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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 peptide Lys-Glu-Trp 

answer the following questions:       

 Draw the structure of this peptide when all the ionizable groups are fully protonated.          

How many ionizable groups does this peptide have? Label the pKa for each of the ionizable groups in the structure above.

 Draw the appropriate titration curve for this peptide on graph paper starting at pH 0 and ending at pH 14. Label the x-axis, y-axis and the pKa

 Determine the overall net charge of this peptide at each full equivalent point (including 0) where the ionizable group is 100% deprotonated. 

 Determine the pI for this peptide.

Determine the average overall net charge of a mixture of this peptide in a solution where pH=8.5. 

TABLE 5.1 Properties of the common amino acids found in proteins Name Abbreviations 1- pk of and 3-letter codes pka of pK₂ of lonizing a-COOH Group a-NH3 Group Side Chain A, Ala 9.7 R, Arg 9.0 N, Asn 8.8 9.8 10.8 9.1 9.7 9.6 9.2 9.7 9.6 9.0 9.2 9.1 10.6 9.2 10.4 9.4 9.1 9.6 Alanine Arginine Asparagine Aspartic acid Cysteine Glutamine Glutamic acid Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine D, Asp C, Cys Q, Gin E, Glu G, Gly H, His I, lle L, Leu K, Lys M, Met F, Phe P, Pro S, Ser T, Thr W, Trp Y, Tyr V, Val 2.3 2.2 2.0 2.1 1.8 2.2 2.2 2.3 1.8 2.4 2.4 2.2 2.3 1.8 2.0 2.2 2.6 2.4 2.2 2.3 12.5 3.9 8.3 - 4.2 6.0 - - 10.0 10.1 -
Transcribed Image Text:TABLE 5.1 Properties of the common amino acids found in proteins Name Abbreviations 1- pk of and 3-letter codes pka of pK₂ of lonizing a-COOH Group a-NH3 Group Side Chain A, Ala 9.7 R, Arg 9.0 N, Asn 8.8 9.8 10.8 9.1 9.7 9.6 9.2 9.7 9.6 9.0 9.2 9.1 10.6 9.2 10.4 9.4 9.1 9.6 Alanine Arginine Asparagine Aspartic acid Cysteine Glutamine Glutamic acid Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine D, Asp C, Cys Q, Gin E, Glu G, Gly H, His I, lle L, Leu K, Lys M, Met F, Phe P, Pro S, Ser T, Thr W, Trp Y, Tyr V, Val 2.3 2.2 2.0 2.1 1.8 2.2 2.2 2.3 1.8 2.4 2.4 2.2 2.3 1.8 2.0 2.2 2.6 2.4 2.2 2.3 12.5 3.9 8.3 - 4.2 6.0 - - 10.0 10.1 -
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