Plant pigments in spinach were separated with TLC with cellulose as the stationary phase and 80/20 diethyl ether/acetone mixture as the mobile phase. If needed, the solvent structures in the lab Appendix. 1. Determine the Rf values of each plant component on this TLC plate. 2. Arrange the plant components from most polar to most nonpolar. Please answer both 1 and 2 (picture below is the same question attached

Plant pigments in spinach were separated with TLC with cellulose as the stationary phase and 80/20 diethyl ether/acetone mixture as the mobile phase. If needed, the solvent structures in the lab Appendix. 1. Determine the Rf values of each plant component on this TLC plate. 2. Arrange the plant components from most polar to most nonpolar. Please answer both 1 and 2 (picture below is the same question attached

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter89: Thin-layer Chromatography

Section: Chapter Questions

Problem 1P

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Similar questions

In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)
Can you DRAW a TLC plate in an elution chamber that shows 3 lanes each with one spot. Label the following in your drawing: Solvent front Origin Mobile phase Stationary phase Distances needed to measure Rf value for one of the spots
A student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only one spot, for which the Rf value is 0.95, is observed. Does this indicate that the unknown material is a pure compound? What can be done to verify the purity of the sample?
If a column was run on the sample shown in lane 3, what would be the first compound to elute off of the column? (assume the same solvent and stationary phase are used) Which of the lanes on this TLC plate show pure compounds? Select all that apply.
A TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ B
A mixture of biphenyl, benzyl alcohol, and benzoic acid is spotted on a silica gel TLC plate and developed using Ethyl Acetate with 0.5% acetic acid as the solvent. Predict the relative Rf (highest to lowest).
How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?
Thin‑layer chromatography (TLC) can be used as a preliminary identification method for many amphetamine‑type stimulants (ATS). Using a 0.25 mm thick silica gel stationary phase and a 98.5:1.5 methanol:concentrated ammonia mobile phase, a spot was observed to have traveled 3.52 cm when the solvent front traveled 8.81 cm. What is the retardation factor (?F)for the compound that composes this spot?
Explain why it is needed to filter the solvent before introducing it to the HPLC system
In HPLC, why is purging or initialization being done before analysis of samples, explain.
An ideal RF value for a compound on a developed TLC plate is 0.30. How could you adjust the TLC developing conditions to obtain an RF of 0.30 if it's too low or too high (Hint: Think about chromatography phases).
Why is it necessary to run TLC in a closed container and to have the interior vapor saturated with the solvent? What error is introduced into the determination of an Rf value if the top is left off of the developing chamber?