Please choose an appropriate technique(s) to separate following compounds (it can be one, more than one, on none) Blue 1 (Food Dye) 792.85 g/mol Blue 2 (Food Dye) 466.36 g/mol None Simple Filtration Column Chromatography Separatory funnel Distilation Crystallization/Re-Crystallization O O 0
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- Which of the following statement is false? a. Component with the strong attraction to the support move more slowly than component with weak attraction. b. Chromatography technique used to separate only colored compounds. c. On TLC, single spot does not mean or guarantee a single substance (compound). d. Iodine vapor and UV- lamp are methods used for visualization uncolored compounds.She performed a normal phase chromatography to separate the components of an extract. She then created another chromatogram using the same stationary and mobile phase to analyze known compounds. The chromatograms are shown below. Which of the following statements is/are TRUE? a. Spot 1 is less polar than Spot 4 b. Compound B is more polar than Compound C c. Spot 3’s identity is Compound B d. Both B & CIdentify these statements with true or false true or false I. Chromatography utilizes partition coefficient theory true or false II. In reverse phase chromatography, the mobile phase is a polar solvent true or false I. Aspirin is an ester prodrug. true or false Aspirin is synthesized by means of an acid catalyzed reaction. true or false I. Grain alcohol boils at 78.3 C true or false Wood alcohol boils at 60 C true or false Diazo coupling involves a sigma and a pi bond in two nitrogen atoms true or false When methyl orange passes through from basic to acidic medium, the change in color is from yellow to intense red true or false Benzoic acid is a white crystalline substance that has a melting point of 122 C true or false Benzoic acid can be prepared by the alkaline reduction of toluene true or false Potassium permanganate is an oxidizing agent true or false Potassium permanganate should never be mixed with glycerin true or false Methyl…
- Two group of students wish to analyse caffeine content in tea samples. Group 1 performed the analysis using thin layer chromatography. Group 2 wish to conduct the analysis using analytical instrumentation. Suggest a possible analytical instrument method for Group 2.Briefly explain the procedural steps before instrumentation. List another two applications of this instrument.Which statement is true? Statement 1: In chromatography, the compound mixture moves along with the mobile phase through the stationary phase and the components get separated depending on the degree of interaction and adhesion Statement 2: Compound A is more soluble in the mobile phase compared to compound B. Thus, compound A would most probably have a higher Rf value.Regarding TLC: a) Compounds A and B are analyzed side-by-side by TLC on silica gel. The developed chromatogram is shown. Which compound is more polar? b) Three unknown mixtures (X, Y, and Z) are analyzed by TLC. The developed plate is shown. What can be concluded about unknown Y? A. Unknown Y contains exactly two different compounds. B. Unknown Y contains at least two different compounds. C. Nothing can be concluded.
- You are assigned to separate a mixture of colourless amino acids present in protein molecule, using chromatography technique. You have a chrome paper (Filter paper sheet), TLC plate, chrome tank or beaker, Solvent (Hexane) and the mixture of amino acids. Answer the following questions related to your experiment. (a). Which of the chromatography technique (paper or thin layer) will give better separation in this experiment?Iman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate the mixture using a column chromatography technique. He packed the column with amino bonded with siloxane material and use a mixture of 20:80 of water: hexane as the mobile phase. i) Sketch the apparatus set up for the chromatographic technique used by Iman. Use the correct chromatographic terminologies to label all of the apparatus, materials, and phases. ii) Predict the sequence of elution when the mixture was loaded onto the separation column. Justify your answer. iii) After the first compound was eluted, Iman discovered that the second compound was taking too long to pass through the column. Please suggest any strategies that could be used to aid the elution process.You were analyzing a mixture of volatile solvents using Gas Chromatography and found out that two peaks are unresolved (not well-separated) using your initial program. Which method can you use to obtain a chromatogram with well separated peaks?
- 4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.5) Given the data of the diet sodas, which one would you recommend to be the healthiest? (You may need to research the functions of caffeine, aspartame and benzoate. Please add references if necessary.)Which is correct? Random errors affect accuracy and systematic errors affect precision. An accurate result shows agreement with the true or accepted value. Precise results can contain significant random error, but little systematic error. The standard deviation is a good measure of accuracy. Polydimethylsiloxane (PDMS) stationary phases have low polarities. Which of the following statements is TRUE about gas chromatography using PDMS stationary phases? The gas chromatography column is typically packed with silica microparticles. An oven is used to control the column temperature because a constant temperature is always needed in gas chromatography Octane (C8H18, boiling point: 126 ºC) is eluted later than dodecane (C12H26, boiling point: 216 ºC) 3-Pentanone ((C2H5)2CO, boiling point: 102 ºC) is eluted later than propanol (CH3CH2CH2OH, boiling point: 97 ºC)Consider the following statements as they apply to gas chromatography. Decide if each is true or false. 1. ___ Temperature programming is necessary only for samples containing compounds with a narrow range of boiling points. 2. ___ GC carrier gases are generally inert gases like helium and nitrogen. 3. ___ The injection port temperature should be set at the average boiling point of all analytes. 4. ___ An FID is a mass-sensitive detector, not a concentration-sensitive device. 5. ___ An air peak is commonly used to determine the dead time when using an FID detector.