Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability (b) for this sample. (assume we started with 100 microliters of cells and added 100 microliters of trypan viability blue). Hemacytometer Grid 1 2 3 456 89 Enlarged view X O O O O O O O C O 8 O O
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- Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in tube-A, then transferred 10 µL to the hemocytometer and started counting cells, then realized the cells were too crowded to count accurately, so Steven took an aliquot of 20 µL cells of cells from tube-A and mixed with 180 µL of cell culture media, transferred 10 µL onto the hemocytometer, and countered 500 cells from the squares on the four corners of the hemacytometer. What is the # of cells per mL? Please show all calculation steps.You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?
- You have an original cell density of 4.3x10 8CF/mL. What is this number in its non-scientific notation or "regular" format?. 43,000,000CF/mL. 0.000000043CfU/mL. 430,000,000CFU/mL. 0.00000043CFU/mL. 4.3CFU/mLWhat kind of cells are suitable for flow cytometric analysis and why? Please answer with 5 dot points/sentences minimumBeginning with 2.0 ×104 cells/L, outline the Evans Blue method for counting viable and dead cells.
- In an experiment to investigate membrane integrity, beet root sections were subjected to different chemicals. Membrane damage was estimated by the color intensity of leaked pigment. Five replicates of each treatment were prepared. Complete the following table with the absorbance values. Calculate the mean (average) and the standard Table 1: Absorbance readings of pigment leaked from damaged cells treated with two different chemicals. Replicate # Absorbance at 460 nm Chemical 2 Chemical 6 1 0.357 0.392 2 0.576 0.395 3 0.334 0.385 4 0.197 0.399 5 0.334 0.403 Average SD Calculate the Coefficient of Variance (see the appendix) for the readings of each chemical: Evaluate the precision of each and state which one is more precise:Why is the plate count method a determination of the number of viable cells?Using the attached image, explain the observed results on the NA and ECM plates. Be sure to address why each sample is either able or unable to grow on each plate and include the genotype(s) of the cells which are capable of growth.
- If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original culture which had been diluted 1x10^-4 times, what is the number of CFU/ml of the original culture?A total of 1,000 cells were counted from 5 squares of the hemocytometer. Of these, 20 are trypan blue-positive cells. What is % non-viable cells? Please show all calculation steps. Your answer (please show all calculation steps):When performing a Standard Plate Count, why are the counts reported as colony-forming units (CFUs) rather than cells?