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When performing a Standard Plate Count, why are the counts reported as colony-forming units (CFUs) rather than cells?
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- Why is the plate count method a determination of the number of viable cells?If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original culture which had been diluted 1x10^-4 times, what is the number of CFU/ml of the original culture?"If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000
- If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test?What are the advantages and disadvantages of the viable plate count method?
- What does a white colony indicate during blue-white screening? Explain how the color is formed.What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?
- For a simple routine qualitative analysis, why is an early morning sample of urine used for the tests, and not a sample collected after a meal?The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?Why is a viable count more sensitive than a microscopic count?What major assumption is made in relating plate count resultsto cell number?