Polymerase chain reaction cannot amplify complete circular plasmids. True False
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- Cosmids are Group of answer choices unmodified Col plasmids unmodified phage. amp-Col plasmids. cos-site-containing plasmids.Conjugation using ________ will result in the transmission of a segment of chromosomal DNAfrom one host to another using a plasmid. Question 25 options: F‑strain High frequency recombination (hfr) strain F+strain F plasmid F' plasmidTrue or False: Plasmids can ONLY be used in transforming bacterial cells.
- True or false double strand rna is synthesized using taq polymerase. plasmids are linear dna moley in a eukaryotic cell dna is packaged into exosomes. Decreasing the concentration of agarose in a gel will allow better separation of smaller fragments of dnaA = double cutter (1000 bp, 800 bp) - 800 is being cut by the single cutter because 600 + 200 = 800, 1000 is intact B = single cutter (2000 bp) A/B (1000 bp, 600 bp, 200 bp) Using the plasmid map identify which enzyme is the single and double cutterSequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) Plasmid
- Which of the following isoforms has faster rate of renaturation given the same concentration? options: Plasmid DNA (with smaller genome size). Chromosome DNA (with bigger genome size). Plasmid and chromosome DNA mixture. None of the above.The function of enzyme involved in base excision repair isa) Addition of correct baseb) Addition of correct nucleotidec) Removal of incorrect based) Removal of phosphodiester bondWhich of the following is NOT true about Base Excision repair? Group of answer choices Ligase is responsible for resealing the nick in the phosphodiester backbone. Endonuclease is used to remove the damaged nucleotide. It is a preferred method of removal for larger defects in the genetic code Polymerase creates the new nucleotide to replace the damaged one Glycosylase removes damaged the damaged base before the nucleotide
- Generally a plasmid vector contains how many elements?a) 1b) 2c) 3d) 4AZT (3'-azido-2',3'-dideoxythymidine) is a drug for HIV infection that gets incorporated into growing viral DNA and blocks the activity of: reverse transcriptase Integrase DNA ligase DNA polymerase β (beta).Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. There are NO sites where multiple enzymes cut. How many base pairs long is the plasmid? 3kb 2kb 4kb 5kb