Possible Unknown Strains: Stapylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Instructions: Your received an email from me with your unknown file. The unknown contains one gram positive and one gram negative strain. You receive a virtual mixed culture containing both of your strains. Use the file you received to answer the following questions: 1. How you would determine the morphology of each strain 2. How you would isolate the two strains from a mixed culture onto separate TSA plates 3. How you would obtain a Gram stain of each individual strain 4. How you would determine which two strains that you have based on the results shown 5. What is your gram positive unknown 6. What is your gram negative unknown

Possible Unknown Strains: Stapylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Instructions: Your received an email from me with your unknown file. The unknown contains one gram positive and one gram negative strain. You receive a virtual mixed culture containing both of your strains. Use the file you received to answer the following questions: 1. How you would determine the morphology of each strain 2. How you would isolate the two strains from a mixed culture onto separate TSA plates 3. How you would obtain a Gram stain of each individual strain 4. How you would determine which two strains that you have based on the results shown 5. What is your gram positive unknown 6. What is your gram negative unknown

Microbiology for Surgical Technologists (MindTap Course List)

2nd Edition

ISBN:9781111306663

Author:Margaret Rodriguez, Paul Price

Publisher:Margaret Rodriguez, Paul Price

Chapter4: The Prokaryotes

Section: Chapter Questions

Problem 4UTM

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Based on the pictures provided, tell me what I have for the gram-positive, and what I have for the gram-negative? Possible Unknown Strains: Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Name one negative and one positive of which I have.
The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?
There are so many microbes in a single mL of culture, it is very difficult to perform one dilution to produce countable cells. Microbiologists need to perform a dilution series, where multiple dilutions are performed in sequence to arrive at the correct dilution. Dilutions are cumulative. Multiple the series of dilutions together to find the final dilution value. If 3 serial dilutions are performed, each with a value of 0.01, what is the cumulative dilution? Express your answer as an exponent, e.g. 0.1 would be 1e-1 and 0.01 would be 1e-2
What is the microbiology laboratory test that Identifies Pseudomonas putida and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.
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Hello, I want you pleaseeee to make a dichotomous key based on provided experiments to identify a an unknown bacterium mixture. It can be E. coli, B. Bulgaris, B subtilis, b aerogenes, m leuteus, s. Aureus, and s epidermisis. Make it based on these possible experiments: phenol red broth, mr/vp test, catalase test, oxidase test, nitrate reduction test, citrate utilization test, Malonate utilization test, decarboxylation test, starch hydrolysis test, dna hydrolysis test, lipid hydrolysis test, gelatin hydrolysis test, urea hydrolysis, SIM medium, triple sugar iron agar test, blood agar test. So far we have run a MAC agar and PEAA plate of the bacterium
Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixture
What is the microbiology laboratory test that Identifies Pseudomonas maumuensis and what are the results of the tests that identify it? please include your sources in MLA. examples are hemolytic tests, lipid concentrations, genomic tests etc.
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Based on the table, what can you conclude from the said data? Title: Indicate whether the microorganism is Resistant, Intermediate or Susceptible with the antibiotic based on the measurement of their zone of inhibition. ANTIBIOTIC MICROORGANISMS Pseudomonas aeruginosa Escherichia coli Staphylococcus aureus Amoxicillin (AMC) Resistant Resistant Resistant Cephalothin (CF) Resistant Susceptible Resistant Chloramphenicol (C) Intermediate Susceptible Resistant Ciprofloxacin (CIP) Susceptible Susceptible Resistant Clindamycin (CC) Resistant Resistant Resistant Erythromycin (E) Resistant Resistant Resistant Oxacillin (OX) Resistant Resistant Resistant Penicillin G (P) Resistant Resistant Resistant Streptomycin (S) Resistant Susceptible Resistant Tetracycline (TE) Resistant Intermediate Resistant Tobramycin (TM) Susceptible…
You have used morphological observations and biochemical tests to identify two unknown bacterial isolates in your practical sessions, briefly describe three other techniques that can be used to identify unknown bacteria (you can use any reliable online resources). Note: These must not be based on biochemical tests (enzyme use, acid production etc) or morphological characteristics (size, shape, structures).
Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________