Predict the effect on the PCR reaction if any of the following circumstances arose: i) there are no primers in the reaction, i) there are no dNTPs in the reaction, iii) there is no Taq polymerase in the reaction, A PCR would not commepce. B. PCR would proceed normally. C. PCR would cease after a few cycles. D. Non-specific DNA amplification will occur.

Predict the effect on the PCR reaction if any of the following circumstances arose: i) there are no primers in the reaction, i) there are no dNTPs in the reaction, iii) there is no Taq polymerase in the reaction, A PCR would not commepce. B. PCR would proceed normally. C. PCR would cease after a few cycles. D. Non-specific DNA amplification will occur.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 12QP

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Describe the possible outcome of a PCR experiment in which (a) there is a single-stranded break in the target DNA sequence, which is present in only one copy in the starting sample, and (b) there is a doublestranded break in the target DNA sequence, which is present in only one copy in the starting sample.
Explain why a positive control and negative control are included in PCR experiments. Explain the three steps involved in each cycle of polymerase chain reaction.Why is loading dye added to the DNA sample for gel electrophoresis? Explain the function of the following components in a PCR reaction:− Primer, dNTP, MgCl, Taq polymerase, buffer.
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