Prepare a standard tube that contains 0.2 mL of 2.5 mM lysine. To this tube, add 0.1 mL of the ninhydrin reagent. What is the concentration of lysine in the final reaction mixture?
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- If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.What is the role of imidazole in Ni-Affinity Chromatography?Is this an drawing of the chemical reaction involved in Lugol’s test?
- In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the Van-Deemter equation.How many moles of KBr are there in 89.5 mL of 0.150 M KBr?Why does eluent strength increase as solvent becomesless polar in reversed-phase chromatography, whereas eluentstrength increases as solvent becomes more polar in normal-phase chromatography? What kind of gradient is used in supercritical fluid chromatography?