Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 μL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 3 (i) (ii) (iii) 2 (iv) 4 o 0 1000 466 © Ø Note: Count cells on the four labelled squares. Include cells touching the line on top and left. O Oe Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.
Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25 flask. After trypsinization, she prepared her cells by mixing 50 μL of the cell suspension with 50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below: 3 (i) (ii) (iii) 2 (iv) 4 o 0 1000 466 © Ø Note: Count cells on the four labelled squares. Include cells touching the line on top and left. O Oe Determine the number of viable and dead cells from her observation. What is the percentage of viability of this culture? Show your calculations in detail. Calculate the concentration of viable cells per mL in the original culture. Show your calculations in detail. Based on your understanding of cell culture, do you think she should proceed with subculture? Justify your answer.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 10SST
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Transcribed Image Text:Prior to subculture, Tifa used a hemocytometer to count her HepG2 cells cultured on a T-25
flask. After trypsinization, she prepared her cells by mixing 50 µL of the cell suspension with
50 μL of 0.4% trypan blue. Her observation under the microscope is as shown below:
1
(1)
(ii)
3
(iii)
2
(iv)
4
Note: Count cells on the four labelled squares. Include cells touching the line on top and left.
18P
06.
¿66
Ⓡ
Determine the number of viable and dead cells from her observation.
What is the percentage of viability of this culture? Show your calculations in detail.
Calculate the concentration of viable cells per mL in the original culture. Show your
calculations in detail.
Based on your understanding of cell culture, do you think she should proceed with
subculture? Justify your answer.
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