Draw a graph based on the result. And give a short discussion based on group1, group 2 and group 3. ProcedureA3 mlAddof50TakemixtureTop up to 50ml.- AddbothultheMixSmlfreshampicillintoovernight culturetoLB -mixtureanalyseOD600with 45 ml LBthebrothThe mixture isthenWithdraw 3ml mixture toMeasure every 15mins. Stop untilOvernight cultureanalyse (OD600)record aszero time-point.incubated atshakedincubator at 37°c 200rpm(E-coli + LB borth)75 mins.Mix5mlTake3mlAdd50ulofovernight culturemixtureNo ampicilintoampicillin totheB (control) with 45 ml LBbrothanalysethemixtureOD600The mixture isthenMeasure every 15mins. Stop untilWithdraw 3ml mixture toanalyse (OD600)record aszero time-point.- incubatedat shakedincubator at 37°c 200rpm75 mins.ResultGroup 2Group 3Group 1(A)(B)(A)(B)(A)(B)OD 600Time (minutes)AmpicilinControlAmpicilinControlAmpicilinControl0.1240.2480.3150.2890.1330.121150.7560.3670.4850.1620.1680.167300.6450.4280.7570.0570.2480.053450.6330.6951.0250.0520.3390.018600.5990.9371.4340.050.5090.053750.5931.3011.7780.0390.6760.053

We can test the antimicrobial resistance of any strain of bacteria by exposing the bacteria to that…

Procedure A Take 3 ml Add 50 ul of Top up to 50ml. - Add fresh LB Mix Sml ampicillin to the overnight culture- mixture to mixture with 45 ml LB analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 Overnight culture - incubated at shaked incubator at 37°c 200rpm - analyse (OD600)record as zero time-point. mins. Stop until (E-coli + LB borth) · 75 mins. Mix overnight culture 5ml Take 3 ml Add 50 ul of ampicillin to mixture No ampicilin mixture analyse OD600 to the B (control) with 45 ml LB broth the Withdraw 3ml mixture to analyse (OD600)record as zero time-point. The mixture is then Measure every 15 mins. Stop until incubated at shaked incubator at 37°c 200rpm 75 mins. Result Group 1 Group 2 Group 3 OD 600 (A) (B) (A) (B) (A) (B) Time (minutes) Ampicilin Control Ampicilin Control Ampicilin Control 0.124 0.248 0.315 0.289 0.133 0.121 15 0.756 0.367 0.485 0.162 0.168 0.167 30 0.645 0.428 0.757 0.057 0.248 0.053 45 0.633 0.695 1.025 0.052 0.339 0.018 60 0.599 0.937 1.434 0.05 0.509 0.053 75 0.593 1.301 1.778 0.039 0.676 0.053

Procedure A Take 3 ml Add 50 ul of Top up to 50ml. - Add fresh LB Mix Sml ampicillin to the overnight culture- mixture to mixture with 45 ml LB analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 Overnight culture - incubated at shaked incubator at 37°c 200rpm - analyse (OD600)record as zero time-point. mins. Stop until (E-coli + LB borth) · 75 mins. Mix overnight culture 5ml Take 3 ml Add 50 ul of ampicillin to mixture No ampicilin mixture analyse OD600 to the B (control) with 45 ml LB broth the Withdraw 3ml mixture to analyse (OD600)record as zero time-point. The mixture is then Measure every 15 mins. Stop until incubated at shaked incubator at 37°c 200rpm 75 mins. Result Group 1 Group 2 Group 3 OD 600 (A) (B) (A) (B) (A) (B) Time (minutes) Ampicilin Control Ampicilin Control Ampicilin Control 0.124 0.248 0.315 0.289 0.133 0.121 15 0.756 0.367 0.485 0.162 0.168 0.167 30 0.645 0.428 0.757 0.057 0.248 0.053 45 0.633 0.695 1.025 0.052 0.339 0.018 60 0.599 0.937 1.434 0.05 0.509 0.053 75 0.593 1.301 1.778 0.039 0.676 0.053

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter29: Examinations And Procedures Of Body Systems

Section: Chapter Questions

Problem 29.3CS

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