Protein Denatured a. Show that the fraction of denatured macromolecules, 0, is related to the equilibrium constant, Kd, by 1+ K. b. At pH=2, the standard enthalpy and entropy of formation for the denaturation of the enzyme chymotrypsin are 418 kJ/mol and 1.32 kJ K-l mol', respectively. Use these data to generate a plot that shows the temperature dependence of 0. Assume the enthalpy and entropy change to be independent of temperature. c. The "melting temperature" of a biological molecule is defined as the temperature at which 0=1/2. Determine the "melting temperature" of chymotrypsin at pH=2.

Protein Denatured a. Show that the fraction of denatured macromolecules, 0, is related to the equilibrium constant, Kd, by 1+ K. b. At pH=2, the standard enthalpy and entropy of formation for the denaturation of the enzyme chymotrypsin are 418 kJ/mol and 1.32 kJ K-l mol', respectively. Use these data to generate a plot that shows the temperature dependence of 0. Assume the enthalpy and entropy change to be independent of temperature. c. The "melting temperature" of a biological molecule is defined as the temperature at which 0=1/2. Determine the "melting temperature" of chymotrypsin at pH=2.

Chemistry: Principles and Reactions

8th Edition

ISBN:9781305079373

Author:William L. Masterton, Cecile N. Hurley

Publisher:William L. Masterton, Cecile N. Hurley

Chapter16: Spontaneity Of Reaction

Section: Chapter Questions

Problem 78QAP

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