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Provide a photograph of an autoclave with labels. Discuss briefly how autoclaving control microbial growth.
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- Microbial products can be classified as three major categories. With the use of sketch, briefly explain the kinetic patterns of growth and product formation in batch fermentation.Provide the conditions (e.g., intrinsic properties, storage conditions, etc.) that will make the recovery of the following microorganisms in commercially sterile foods acceptable: a) Obligate thermophilic sporeforming bacteria b) Acid-tolerant microorganisms c) Mesophilic or thermophilic sporeformersDescribe the advantages and disadvantages of aldehyde agents in microbial control.
- Why does a precipitate form on Sierra’s medium when inoculated with a lipolytic organism? Discuss the principle behind this procedure.Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.In an autoclave, how are temperatures above boiling achieved? (Answer in not more than 2 sentences)
- What is sterilization? Give specific examples of media which are sterilized by the following methods:A. Boiling or heating onlyB. Autoclave using steam under pressure.C. InspissatorWhat is the point of the pressure in the autoclave sterilization?Discuss the benefit and drawbacks of using cell suspension culture for the production of important secondary metabolites.
- Explain the advantages of fed-batch operation mode and give an example for a type of product obtained by fed-batch bioprocess.What type of media components cannot be sterilized in an autoclave?Discuss briefly the phases of Microbial Growth Curve and why it is useful to plot the microbial growth from a growing microbial culture?