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Q: The standard temperature-pressure combination for an autoclave isa. 100°C and 4 psi b. 121°C and 15…
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In an autoclave, how are temperatures above boiling achieved? (Answer in not more than 2 sentences)
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- What is the point of the pressure in the autoclave sterilization?What is the effect of moist heat and dry heat? Define TDT and TDP. What is the minimum sterilization condition of autoclave? What is tyndallization, incineration, pasteurization.placing bacteria at ultracold temperatures is as effective at killing microbes as the use of dry heat in incineration. is is true/false?
- Why do boiled and unboiled potato extracts differ in their action on hydrogen peroxide? Explain in 1-3 sentencesWhich technique is preferred in the separation of fatty acyl methyl esters from a mixture?a) Gas-liquid chromatographyb) Absorption chromatographyc) TLCd) CentrifugationThe standard temperature-pressure combination for an autoclave isa. 100°C and 4 psi b. 121°C and 15 psi c. 131°C and 9 psi d. 115°C and 3 psi
- In autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and pressure at 15 psi for 15 to 20 min? What will happen if this was done in only for 10 min? Can you autoclave lab materials and decontaminate cultures at the same time?Identify the oxidizing agent in the following REDOX reaction? CI2 + NaBr --> NaCI + Br2 A) NaCI B) NaBr C) CI2 D) Br2Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?
- Two cultures of a facultative anaerobe are grown in the same medium, but one culture is exposed to air and the other is maintained under anaerobic conditions. Which of the two cultures will contain more cells at the end of a week? Why?In the traditional alkaline lysis method of Solution 1, what is the purpose of Glucosel?An autoclave is typically used for sterilization of media. a. Define sterilization. b. Under what conditions are media typically sterilized in an autoclave? c. What type of media components cannot be sterilized in an autoclave? d. What is an alternative to autoclaving for sterilizing heat-sensitive materials?