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- Q6) Why are ‘sticky ends’ useful biotechnologically? Q7) Why is this enzyme called a restriction endonuclease? How is it different from an exonuclease?Q.1. Imagine you are working for a research laboratory and you are asked to help withthe following:a. Cut the following segment of DNA using E. Co R I. Write the sequence offragments clearly and separately. ATTTACGAATTCTTCCAAGAATTCCTAAATGCTTAAGAAGGTTCTTAAGG b. Show sticky ends? How restriction endonuclease are different from the restriction-modification system?Provide a brief summary of the Sanger sequencing method.
- 13. Which of the following techniques did not provide strong evidence that DNA is the genetic material? labeling of bacteriophage DNA with 32P labeling of bacteriophage protein with 35S treatment of heat killed Streptococcus pneumoniae cell components with various enzymes transformation of R form Streptococcus pneumoniae with heat killed S form S. pneumoniae preparations42. What is the distinction between plectonemic and solenoidal supercoiling? Which of the two is more stable in the absence of protein? Which form of supercoiling is found within cells and why?40. What is the relationship between the free energy of supercoiling and the superhelical density? How does it create a constraint on the extent of supercoiling? How does supercoiling facilitate the formation of alternative DNA structures? Do organisms overwind or underwind their genomes?
- 10. DNA clones, derived via reverse transcription of mRNA molecules into DNA and cloned into a vector form a ?11-3 Use his- strand bacteria describe an Ames test and list hypothetical outcomes for these compounds: Compound 1 – safe to organisms Compound 2 – causes base-pair substations Compound 3 – causes frameshift mutations Compound 4 - causes both base-pair substations and frameshift mutationsWhy is so much effort being poured into genome mapping applications?
- 30. Which of the following is FALSE when comparing RNA and DNA? A.are composed of nucleotides that contain a phosphate group, a pentose sugar, and a nitrogenous base. B. Both contain nucleotides connected by phosphodiester linkages. C. Both have a 5’ end and a 3’ end. D. Both are composed of identical nucleotides.1a) When performing classical Sanger or "dideoxy" sequencing, you set up 4 parallel reactions per template to be sequenced from a specific primer, with each of the four reactions containing a different dideoxynucleotide, and then the four reactions were run in a separate, adjacent lanes on a gel. Why couldn't you combine all 4 dideoxynucleotides with the primer and the template and do the whole reaction in one tube, and then run the set of fragments produced by the reaction mixture on a single lane in an acrylamide gel?26. How does the flu virus gain entry into cells? (MARK ALL THAT APPLY) Group of answer choices by slipping through carbohydrate import machinery by cleaving the glycosidic bonds by unloading their genetic material onto extracellular glycoproteins by attaching to ion channels by binding to glycoproteins on the cell surface that are unique to specific cells