Doc -2 Therapeutic Drugs Doc-1-Introduction: Specific applicati ons of genetic engineening are abundant & increase rapidly especially in production of medications (pharmaceuticals proteins). The application of recombinant proteins in medications minimizes the risk of transmitting infections which used to happen in the past upon consuming proteins purified from animal tissues. Recombinant proteins are safer as well because unlike animal proteins, they are not recognized as foreign molecules by the immune system, and as a result they don't cause all ergy. In this lesson, we will identify the method of insulin production for therapeutic use. Doc-2- Insulin Production Insulin is a protein functions in reducing the glucose level in the blood In some patients, there is deficiency in insulin, so they depend on exogenous insulin to control their blood glucose level. In past, scientists used anim als like horses & rabbits to produce insulin, this caused allergy in some cases & it was expensive, moreover it was being produced in low amounts. Nowadays, thanks for biotechnol ogy. insulin is produced in high amounts, in less time, in low costs and with no nisk for any allergy. The applied experimental procedure in pro ducing recombinant proteins is the same one adapted for gene cloning except that the gene of interest is now specified. a- Translate the procedure into a text. The plasmid is extracted and then cleaved with restriction enzyme The insulin producing bacteria are cultured for proliferation E.coli C The plasmid is reintroduced to obtain recombinant bacteria The insulin gene is inserted into the plasmid Insulin is isolated and purified from the bacteria The insulin gene is isolated from a human cell Production of protein ( Human insulin) by Genetic Engincering The insulin is administered to a patient with diabetes Dac-3- Advantages (Benefts): L Economic Value - It is produced in high am ounts. - It is produced in less time. - It is less expensive (low cost). Medical Value - It is used as a medicati on by diabetic patients. - It is healthy, safe & doesn't cause allergy. 130

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy
Section: Chapter Questions
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BaGuila 12 FE/LH-Vsias'-OBaram kmail-EadL maa
Doc -2
Therapeutic Drugs
> Doc-1-Introduction:
Specific applicati ons of genetic engineering are abundant &
increase rapidly especially in production of medications
(pharmaceuticals proteins).
The application of recombinant proteins in medications
minimizes the risk of transmitting infections which used to
happen in the past upon consuming proteins purified from animal
tissues. Recom binant proteins are safer as well because unlike
animal proteins, they are not recognized as foreign molecules by
the immune system, and as a result they don't cause allergy.
In this lesson, we will identify the method of insulin producti on
for therapeutic use.
> Doc-2- Insulin Production
Insulin is
reducing the glucose level in the
blood.
a protein functions in
The plasmid is extracted
and then cleaved
with restriction enzyme
The insulin
producing bacteria
are cultured for
proliferation
In some patients, there is deficiency in
insulin, so they depend on exogenous
insulin to control their blood glucose
level. In past, scientists used animals
like horses & rabbits to produce
insulin, this caused allergy in some
cases & it was expensive, moreover it
was being produced in low amounts.
Nowadays, thanks for biotechnology,
insulin is produced in high amounts, in
less time, in low costs and with no nisk
for any allergy.
The applied experimental procedure in
producing recombinant proteins is the
same one adapted for gene cloning except that the gene of interest is now specified.
a- Translate the procedure into a text.
E.coli
The plasmid is reintroduced
to obtain
The insulin
gene is inserted
into the piasmid
recombinant
bacteria
Insulin is isolated
and punfied from
the bacteria
The insulin
gene is
isolated from
a human cel
Production of protein ( Human insulin)
by Genetie Engineering
The insulin is
administered
to a patient
with diabetes
> Dac-3- Advantages (Bene fits):
1 Ecunomic Value
- It is produced in high am ounts.
- It is produced in less time.
-It is less expensive (low cost
2 Medical Value
-It is used as a medicati on by diabetic patients.
- It is healthy, safe & doesn't cause allergy.
130
Transcribed Image Text:BaGuila 12 FE/LH-Vsias'-OBaram kmail-EadL maa Doc -2 Therapeutic Drugs > Doc-1-Introduction: Specific applicati ons of genetic engineering are abundant & increase rapidly especially in production of medications (pharmaceuticals proteins). The application of recombinant proteins in medications minimizes the risk of transmitting infections which used to happen in the past upon consuming proteins purified from animal tissues. Recom binant proteins are safer as well because unlike animal proteins, they are not recognized as foreign molecules by the immune system, and as a result they don't cause allergy. In this lesson, we will identify the method of insulin producti on for therapeutic use. > Doc-2- Insulin Production Insulin is reducing the glucose level in the blood. a protein functions in The plasmid is extracted and then cleaved with restriction enzyme The insulin producing bacteria are cultured for proliferation In some patients, there is deficiency in insulin, so they depend on exogenous insulin to control their blood glucose level. In past, scientists used animals like horses & rabbits to produce insulin, this caused allergy in some cases & it was expensive, moreover it was being produced in low amounts. Nowadays, thanks for biotechnology, insulin is produced in high amounts, in less time, in low costs and with no nisk for any allergy. The applied experimental procedure in producing recombinant proteins is the same one adapted for gene cloning except that the gene of interest is now specified. a- Translate the procedure into a text. E.coli The plasmid is reintroduced to obtain The insulin gene is inserted into the piasmid recombinant bacteria Insulin is isolated and punfied from the bacteria The insulin gene is isolated from a human cel Production of protein ( Human insulin) by Genetie Engineering The insulin is administered to a patient with diabetes > Dac-3- Advantages (Bene fits): 1 Ecunomic Value - It is produced in high am ounts. - It is produced in less time. -It is less expensive (low cost 2 Medical Value -It is used as a medicati on by diabetic patients. - It is healthy, safe & doesn't cause allergy. 130
BioGuide 12EE/LH - Venion 8"- O Bassam Ianad - Rand Morna
Doc -2
• The Transfer of Genes
> Doc-0-Int roduction:
Transgenesis is the process of transfer of genes from one organism into another of
same or different species in order to produce a new organism with significant
economic & nutritional values.
> Doc-1-Transgensis in Plants
agrobacterium
plasmid
possessing a gene responsible for
infecting plant roots causing
tumor. This plasmid is extracted
and the mentioned gene is
removed and thus, the plasmid is
1-
The
(1)
(2)
(3) Plant
tumefaciens
has
Agro-bacterium TumeFaciens
Bacillus Thuringiensis Bacteria
(It can infect plant root cells to cause tumor) (it contains an insecticide gene)
(insecticide gene is a
gene that produces a
toxic protein against
some insects)
Plasmid
linearized.
Tumoral
insecticide
genes
removed
gene
2- On the other hand, another
as bacillus
bacterium known
Gene
graft
thuringenesis has a gene that
controls the synthesis of a toxic
protein against some insects. The
latter gene is isolated and grafted
or ligated in the linearized
agrobacterium plasmid to obtain
the recombinant plasmid.
insert the
recombinant plasmid
into
Plant cell
the agrobacterium
Protoplast
(with no cell wall)
This bacteria
infect
protoplast by
electric shock
1+2- The recombinant plasmid is
reinserted into the agrobacterium
which initial plasmid is removed.
Plant
synthesize
its own
(5)
insecticide
3- On the other hand, a plant cell
is removed from a certain plant,
and its cell wall is removed to
(6)
a Young plant
is regenerated
Transgenic plant
Protoplast in culture
obtain a protoplast.
4 Then, the agrobacterium with the recombinant plasmid infects the protoplast.
5+6- Finally, the protoplast grows into an adult plant that is able to
produce its own insecticide (produce toxins against insects)
LOOKING
FANTASTIC AS
EVER CAROL!
THANKS
GIRLS!
> Doc-li-Advantages
[This plant is known as genetically modified plant or genetically
modified organism (GMO)].
1-There are transgenic maize plants that are resistant to
devastating moths. Thus, farmers don't use insecticides upon
cultivation of such genetically modified plants.
(Insecticides added expenses and are bad for health and the environment).
dirkte
Genetically
Modifled i
heard
140
Transcribed Image Text:BioGuide 12EE/LH - Venion 8"- O Bassam Ianad - Rand Morna Doc -2 • The Transfer of Genes > Doc-0-Int roduction: Transgenesis is the process of transfer of genes from one organism into another of same or different species in order to produce a new organism with significant economic & nutritional values. > Doc-1-Transgensis in Plants agrobacterium plasmid possessing a gene responsible for infecting plant roots causing tumor. This plasmid is extracted and the mentioned gene is removed and thus, the plasmid is 1- The (1) (2) (3) Plant tumefaciens has Agro-bacterium TumeFaciens Bacillus Thuringiensis Bacteria (It can infect plant root cells to cause tumor) (it contains an insecticide gene) (insecticide gene is a gene that produces a toxic protein against some insects) Plasmid linearized. Tumoral insecticide genes removed gene 2- On the other hand, another as bacillus bacterium known Gene graft thuringenesis has a gene that controls the synthesis of a toxic protein against some insects. The latter gene is isolated and grafted or ligated in the linearized agrobacterium plasmid to obtain the recombinant plasmid. insert the recombinant plasmid into Plant cell the agrobacterium Protoplast (with no cell wall) This bacteria infect protoplast by electric shock 1+2- The recombinant plasmid is reinserted into the agrobacterium which initial plasmid is removed. Plant synthesize its own (5) insecticide 3- On the other hand, a plant cell is removed from a certain plant, and its cell wall is removed to (6) a Young plant is regenerated Transgenic plant Protoplast in culture obtain a protoplast. 4 Then, the agrobacterium with the recombinant plasmid infects the protoplast. 5+6- Finally, the protoplast grows into an adult plant that is able to produce its own insecticide (produce toxins against insects) LOOKING FANTASTIC AS EVER CAROL! THANKS GIRLS! > Doc-li-Advantages [This plant is known as genetically modified plant or genetically modified organism (GMO)]. 1-There are transgenic maize plants that are resistant to devastating moths. Thus, farmers don't use insecticides upon cultivation of such genetically modified plants. (Insecticides added expenses and are bad for health and the environment). dirkte Genetically Modifled i heard 140
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