Q.A protein has two domains, which are labeled with two probes for energy transfer. Calculate the distance between two domains of the protein, if the steady state fluorescence intensities of donor- labeled protein and donor-acceptor labeled protein are 1000 and 250 respectively [Ro is given as 40 Á].

Biochemistry
9th Edition
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Chapter7: The Behavior Of Proteins: Enzymes, Mechanisms, And Control
Section: Chapter Questions
Problem 13RE: REFLECT AND APPLY Explain the experiment used to determine the structure of ATCase. What happens to...
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Q.A protein has two domains, which are labeled
with two probes for energy transfer. Calculate the
distance between two domains of the protein, if
the steady state fluorescence intensities of donor-
labeled protein and donor-acceptor labeled protein
are 1000 and 250 respectively [Ro is given as 40 Á].
Transcribed Image Text:Q.A protein has two domains, which are labeled with two probes for energy transfer. Calculate the distance between two domains of the protein, if the steady state fluorescence intensities of donor- labeled protein and donor-acceptor labeled protein are 1000 and 250 respectively [Ro is given as 40 Á].
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