QUESTION 2 a) Figure 4 shows effect of catalyst on rate of reaction. Energy Products Reactants Reaction progress Figure 4 i) Write letter M and N in the box given. M to indicate activation energy without enzyme and N to indicate activation energy with enzyme. i) State two (2) ways on how enzyme lowers activation energy (E). ......... .............
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
![c) Figure 5 shows competitive and non-competitive inhibition.
V max
D
KM
[S]
Figure 5
i)
Which labelled graph represents competitive and non-competitive inhibition?
Competitive inhibition:
Non-competitive inhibition:
ii) Which labelled graph has the highest KM value?
iii) State the advantage of having low KM value.
d) Explain how sarin molecules inhibit acetylcholinesterase.
e) Which method of enzyme immobilisation is used to produce lactose free milk?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F835f8ff3-547d-45b5-90f8-c0a9d4c5a6ed%2F4eda2b74-d4fd-4203-8121-095b1ffbf6f3%2F58v3kh_processed.jpeg&w=3840&q=75)
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