3. (a) Comparing the culture-dependant (e.g., colony forming units count) and DNA-based (e.g., PCR) bacterial number quantification methods, what are the primarydisadvantages of using culture-dependent methods to quantify bacterial numbers in anenvironmental sample? Use no more than three sentences or bullet points to presentyour answer succinctly. (b) At a local wastewater treatment plant receiving municipal wastewater, thesecondary treatment units have been performing severely under the designedbenchmarks, resulting in poor effluent quality. You, a wastewater treatment engineer,suspected that the wastewater treatment microbes in the units might be inhibited.What factors do you want to check to find out potential causalities of the inhibition?List the most common four factors that you want to start your investigation with.e(ii)(iii)_(iv).

Wastewater treatment is the process of removing contaminants and pollutants from wastewater to make…

Answered: 3. (a) Comparing the culture-dependant…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating") bacteria, and what can we use them for? Check all that apply A. To observe colony morphology/color B. To isolate viral plaques C. testing growth conditions or treatments (such as media, antibiotics, incubation temperature etc.,) D. To isolate a clonal population of bacteria from the rest of the culture E. For subculturing (transferring to new media so we can grow more bacteria, storing the plate in a fridge for repeated use so the bacteria don't die quickly) F. Accurate quantification (to calculate CFU/mL) G. To know what strain of bacteria you're working with, since you can tell based on how it looks on the plate. H. To lower risk of contamination/identify contamination
A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
1) What are all of the different colony types that can be seen on a mixed-culture streak plate? 2) What kinds of colonies can be seen when mixed cultures of E. coli, M. luteus, and Serratia marcesens? 3) What is the range of colonies that may be seen with each mixed culture?
1- Why was the T4 and E.coli mixed together in top agar and then poured on the plate instead of just spreading the two together with a glass spreader on the plate? 2- When doing the titration and isolation of the T4 phage, E.coli diluted for you to 10e7 cells/ml. why was a diluted culture used? 3- After doing the titration of T4 phage, you find your dilution plates are all TNTC. how could this have happened? 4- Could you isolate phages that infect E.coli from raw sewerage? explain.
You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate method what other methods could you use to separate the bacteria? Please state what methods you would use, the result you would be looking for and interpret the result.
Briefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the multiplex polymerase chain reaction test (PCR). Which of the tests had the highest accuracy and what methodology and techniques is used for Multiplex PCR test.
PCR tests are becoming more prevalent as a standard for testing for bacterial or viral disease. Describe how PCR would be used in place of a growth culture for Strep throat and why this would be advantageous.
Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *
A culture of E. coli is diluted as follows:(1) 65mL are added to 435mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-3 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?c) How many milliliters of water is needed to make a 10-3 dilution using 1000 uL from the original culture?
During the Lederberg and Tatum experiment, theresearchers compared the growth of mutant strains under twoscenarios: mixed strains or unmixed strains. When the unmixedstrains were plated on the experimental growth medium, whywere no colonies observed to grow? When the mixed strainswere plated on the experimental growth medium, 10 colonieswere observed. What was the significance of these colonies?
you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each other from this mixed culture?( Besides using plate technique)
1.What colony characterisitics can be used for differentiation of bacterial species? As an example, compare the properties of colonies of Serratia marcescens and Micrococcus luteus on your streak plate. 2.Define the term colony as it relates to bacterial growth on solid media? 3. why is dilution a necessary part of pure culture preperation? 4.What advantage(s) does the streak-plate method have over the pour-plate method?

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