QUESTION 26 In hydrophobic interaction chromatography (HIC), which separates proteins based on their hydrophobicity, the bound proteins are eluted by proteins are eluted by Choose a number from the choices shown below. In the case of ion-exchange chromatography, the bound For exclusion chromatography we use 1. increasing NaCl concentration of the buffer. 2. decreasing NaClI concentration of the buffer. 3. increasing acetonitrile concentration of the buffer. 4. decreasing acetonitrile concentration of the buffer. 5. increasing pH of the buffer. 6. decreasing pH of the buffer. 7. none of the above

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QUESTION 26
In hydrophobic interaction chromatography (HIC), which separates proteins based on their hydrophobicity, the
bound proteins are eluted by
In the case of ion-exchange chromatography, the bound
For exclusion chromatography we use
proteins are eluted by
Choose a number from the choices shown below.
1. increasing NaCl concentration of the buffer.
2. decreasing NaCl concentration of the buffer.
3. increasing acetonitrile concentration of the buffer.
4. decreasing acetonitrile concentration of the buffer.
5. increasing pH of the buffer.
6. decreasing pH of the buffer.
7. none of the above
Transcribed Image Text:QUESTION 26 In hydrophobic interaction chromatography (HIC), which separates proteins based on their hydrophobicity, the bound proteins are eluted by In the case of ion-exchange chromatography, the bound For exclusion chromatography we use proteins are eluted by Choose a number from the choices shown below. 1. increasing NaCl concentration of the buffer. 2. decreasing NaCl concentration of the buffer. 3. increasing acetonitrile concentration of the buffer. 4. decreasing acetonitrile concentration of the buffer. 5. increasing pH of the buffer. 6. decreasing pH of the buffer. 7. none of the above
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