QUESTION 27 If each of the following compounds were placed into a column of silica gel for liquid chromatography, which compound would elute first 2 Pentanol 1,3 Dibromobenzene, tert pentylcyclohexane, benzoic acid 20GMU 2020 O Benzoic acid O 1,3-Dibromobenzene O2-pentanol O Tert-pentylcyclohexane
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- If a 0.0920 g sample of a 1:1 mixture of 4-nitroaniline and 2-nitroaniline was separated by column chromatography, and the recovered fractions weighed 0.0191 g ( 4-nitroaniline) and 0.0200 g (2-nitroaniline), what was the percent recovery of these two isomers? 20.6% 43.5% 21.7% 42.4%23-24 A 1.013-g sample of tuna was analyzed by the Kjeldahl method. The ammonia formed was distilled into a 75.00-mL 0.02139 M H₂SO₄ solution. A 5.21 mL back-titration of 0.05324 M NaOH was required. (Note the stoichiometry of your acid and base) QUESTION.a) What is the % N (14.007 g/mol) of the tuna sample? b) If fish in general has 5.71 g protein per g N, what is the % protein of the sample?Multistep synthesis of Lidocaine Lab 1) How many grams of starting material do you need? It is given in moles below but how many grams? 2) How to calculate theoretical yield? Part One Method A. Preparation of alpha-Chloro-2,6-dimethylacetanilideCombine 0.033 mol of 2,6-dimethylaniline with 25 mL of glacial acetic acid (or ethyl acetateor THF) and 0.033 mol of alpha-chloroacetyl chloride, in that order, in an appropriately sized Erlenmeyer flask. With the aid of a hot water bath, warm the solution to 40-50°C, remove the flask from the bath, and add a solution of 5 g of sodium acetate trihydrate dissolved in 100 mL of water.
- 1. Identify the products on your GC chromatogram using the Rf values of the compoundscompared the Rf of the standards.2. Calculate the percent composition for each compound using a ruler to measure.Q5: If the Ksp of Ca3N2 is 6.06 x 10-3, how many moles of calcium nitride (s) could you dissolve in 1.589 L of solvent (assume that calcium nitride (s) dissolves in water to form Ca2+(aq) and N3- (aq))? Enter your answer with at least 3 sig figs. Please provide only typed answer solution no handwritten solution needed allowedRegarding TLC: a) Compounds A and B are analyzed side-by-side by TLC on silica gel. The developed chromatogram is shown. Which compound is more polar? b) Three unknown mixtures (X, Y, and Z) are analyzed by TLC. The developed plate is shown. What can be concluded about unknown Y? A. Unknown Y contains exactly two different compounds. B. Unknown Y contains at least two different compounds. C. Nothing can be concluded.
- Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of your answers on the spots in the chromatogram shown at the right.CATION CONFIRMATORY TEST OBSERVATION ADDED REAGENT(S) OBSERVATIONS Excess NH3 NVR KSCN NVR K4Fe(CN)6 NVR K3Fe(CN)6 NVR Na2C2O4 NVR NaOH (in evaporating dish) Litmus paper: red to blue ANION CONFIRMATORY TEST OBSERVATION ADDED REAGENT(S) OBSERVATIONS HNO3, Fe(NO3)3 Blood red aqueous layer; colorless toluene layer HNO3, KMnO4 Peach aqueous layer; colorless toluene layer (to the ppt) CH3COOH White ppt does not dissolve MgCl2 NVR 6M H2SO4, FeSO4, 18M H2SO4 NVR Select one or more: NH4+ CO32- Fe3+ SO42- Cu2+ NO3- Ca2+ I- Br- PO43- Zn2+ SCN-sample question 2. During further studies on transferrin, the protein was treated with dithiothreitol, then incubated with ICH2CO2H. After this trypsin was added and the products were separated using reversed phase liquid chromatography before being analysed by MALDI mass spectrometry using an a-cyano-4-hydroxycinnamic acid matrix. (i) How would you expect the structure of the protein to be modified by each of the reagents dithiothreitol, ICH2CO2H and trypsin? (ii) Identify two features of a-cyano-4-hydroxycinnamic acid that make it a good MALDI matrix.
- How does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations, leading to the evolution of colored species? Context: Paper chromatography experiment (separation of inorganic cations) with a 9:1 acetone/ HCl solventA 0.9092 g sample of wheat flour was analyzed by the Kjeldahl procedure. The ammonia formed was distilled into 50.00 mL of 0.02532 M H2SO4. A 7.46 mL back-titration of 0.04917 M NaOH was required. (Note the stoichiometry of your acid and base) Calculate the a. %N (FM=14.007) in the sample, b. %Protein in the sample (factor = 5.70)29. What exactly is relative retention and retention time? How do you solve problems like this?