Given the following Western blot results showing levels of proteins over time in three yeast cell extracts after the induction of high level expression of the CDH1 protein.WTGAL-CDH1GAL-CDH1,cdc23-10 30 60 120 0 30 60 120 0 30 60 120 Time (min)| Pds1- Clb2- Ase1- Kar2Note: The GAL-CDH1 indicates that the yeast strain contains a transgenic cdh1 gene under the control of the GAL promoter. This means that the researcher can control expression of that cdh1 gene. In these three experiments (three yeast strains), time O is the point at which GAL-controlled genes begin to get expressed (WT lacks transgenes).which is a component of the AIn this experiment, four proteins are being probed for in three different yeast strains (1. wild-type, 2. a strain containing the cdh1 gene under GAL control (see note), and 3. the GAL-CDH1 strain that is also harboring a cdc23 loss of function allele (alleleThe upper (larger) band in the Ase1 lanes is non-specific background band; disregard it.Which of the following interpretations about these results is the most correct?O The APC is not involved in the degradation of Cib2 and Ase1O Cdh1 is sufficient for Pds1 degradation.O Cdh1necessary for Clb2 degradation.O Cdh1 is sufficient for Clb2 degradation.QUESTION 37Which describes the cell's cortex?O the region of cytoplasm just inside the plasma membrane that is rich in actin, actin-binding proteins and myosinsO the nucleoplasm just inside the nuclear envelope that is rich in nuclear laminsO the proteins around the centriole, including gamma-tubulin ring complexesO the region around the MTOC where it is closely associated with the trans face of the Golgi apparatus

Animal cells have a cell cortex that regulates cell shape.

QUESTION 37 Which describes the cell's cortex? O the region of cytoplasm just inside the plasma membrane that is rich in actin, actin-binding proteins and myosins O the nucleoplasm just inside the nuclear envelope that is rich in nuclear lamins the proteins around the centriole, including gamma-tubulin ring complexes O the region around the MTOC where it is closely associated with the trans face of the Golgi apparatus

QUESTION 37 Which describes the cell's cortex? O the region of cytoplasm just inside the plasma membrane that is rich in actin, actin-binding proteins and myosins O the nucleoplasm just inside the nuclear envelope that is rich in nuclear lamins the proteins around the centriole, including gamma-tubulin ring complexes O the region around the MTOC where it is closely associated with the trans face of the Golgi apparatus

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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. Suppose you have six strains of E. coli. One is wildtype, and each of the other five has a single one of thefollowing mutations: lacZ−, lacY−, lacI−, oc, andlacIS. For each of these six strains, describe thephenotype you would observe using the following assays. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expressionbut cannot serve as a carbon source for bacterialgrowth because it cannot be cleaved byβ-galactosidase; (2) X-gal cannot serve as a carbonsource for growth; (3) E. coli requires active lactosepermease (the product of lacY) to allow lactose,X-gal, or IPTG into the cells.]a. Growth on medium in which the only carbonsource was lactose.b. Colony color in medium containing glycerol as theonly carbon source, X-gal, and IPTG
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Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?