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- Question 1. Suppose that the diagram below represents the genomic organization of an enzyme involved in eye pigment production in mice. Within the gene are four exons. Biochemical analysis has revealed that the active site of the enzyme is located in the C terminus of the protein. The nucleotide length of each exon and intron is shown. The dinucleotide sequence GT represents the 5’ splice site and the dinucleotide sequence AG represents the 3’ splice site. Both the 5’ and the 3’ splice sites must be present for splicing to occur. Assume that the first and second stop codons are located immediately after the first and second 5’ splice sites, respectively; the third and fourth stop codons are located near the 3’ end of exons 3 and 4, respectively; all these stop codons are in the correct reading frame. E) Suppose you isolate a mutant mouse that has white eyes. When you examine the size of the eye pigment enzyme produced by this mouse, you see that it is 400 amino acids long. Sequence…Question 4. If you were to synthesize a tetrapeptide consisting of all alanine residues, you would have how many bonds between carbon and nitrogen?Question 16 Which of the following is not considered as a requirements for the effective binding and translocation of proteins into the ER? signal sequence in the newly synthesized polypeptide GDP SRP and SRP receptor Translocon
- Question 10. All of the following areparts of a nucleotide EXCEPT:A. a five-carbon sugar.B. a six-carbon sugar.C. a phosphate group.D. a nitrogenous base.Question 7: At third codon positions, approximately what proportion of muations are synonymous? A. None B. 4% C.30% D. 70%Question 1 I . Protein Synthesis – complete the chart for this coding region of a gene. DNA sense ATG AAA CGA GTT ACC GAA ACT TAA DNA nonsense mRNA codon tRNA anticodon Amino acid
- QUESTION 13 Where does transcription occur in eukaryotic cells? in the nucleus in the cytoplasm at the golgi apparatus at the plasma membraneQuestion 2: Part a: Complete the table describing different components of intron removal from mRNA. Nu:, X and Y refer to B-type chemistry shown on the previous page. (YELLOW table shown) Part b: Complete the table describing different components of group I self-splicing intron removal from 26S rRNA in Tetrahymena. (BLUE table shown) Part c: Draw the intron with an all atom structure for Branchpoint A after intron removal from mRNA Part d: Draw the Group I self-splicing intron with an all atom structure for the Guanosine cofactor after intron removal from 26S rRNA in Tetrahymena.question 38 The constant region of each heavy chain determines the class of the heavy chain. True False
- QUESTION 22 Which type of mutation results in no change in amino acid sequence for the protein? Silent Missense Nonsense FrameshiftQUESTION 15 Which of the following best explains why each codon only codes for a very specific amino acid? The genetic code is not ambiguous The genetic code is redundant, or degenerate There are different reading frames RNA polymerase makes mistakesQUESTION 22 The DNA sequences that are most conserved between human and mouse would most likely be located in: A Highly-repeated sequences, such as microsatellite regions B Highly repeated sequences, such as Alu sequences C Moderately-repeated non-coding sequences D Coding regions of single-copy genes