Rank from highest enzyme efficiency to lowest enzyme efficiency. To rank items as equivalent, overlap them. All of the kinetics work you performed in Parts F through P has allowed you to determine the values of kcat and KM of your enzyme for the substrate amoxicillin. You repeat all of these experiments with a series of additional ß-lactam substrates, hoping to determine which of the B-lactams are more or less susceptible to hydrolysis by this newly discovered B-lactamase enzyme.kicat(s)Км(uM)AntibioticAmoxicillin130Cephalothin1,0006,000Cefotaxime147,900Ceftazidime2.520,000Aztreonam3.55,000Imipenem0.092.9"Data based on published data for GES-14 in Table 2 from Delbrück H, Bogaerts P, Kupper MB, Rezende de Castro R, Bennink S, Glupczynski Y, Galleni M, Hoffmann KM, Bebrone C. Kinetic and crystallographic studies of extended-spectrum GES-11, GES-12, and GES-14B-lactamases. Antimicrob Agents Chemother. 2012 Nov:56(11):5618. ResetHelpcephalothinamoxicillinaztreonamceftazidimeimipenemcefotaximeBest substrate (highest enzyme efficiency)Worst substrate (lowest enzyme efficiency)O The correct ranking cannot be determined.

The turnover number or Kcat is the maximum number of chemical conversions of substrate molecules…

Answered: Rank from highest enzyme efficiency to…

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Biochemistry

Rank from highest enzyme efficiency to lowest enzyme efficiency.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Rank from highest enzyme efficiency to lowest enzyme efficiency. To rank items as equivalent, overlap them.

All of the kinetics work you performed in Parts F through P has allowed you to determine the values of kcat and KM of your enzyme for the substrate amoxicillin. You repeat all of these experiments with a series of additional ß-
lactam substrates, hoping to determine which of the B-lactams are more or less susceptible to hydrolysis by this newly discovered B-lactamase enzyme.
kicat
(s)
Км
(uM)
Antibiotic
Amoxicillin
130
Cephalothin
1,000
6,000
Cefotaxime
14
7,900
Ceftazidime
2.5
20,000
Aztreonam
3.5
5,000
Imipenem
0.09
2.9
"Data based on published data for GES-14 in Table 2 from Delbrück H, Bogaerts P, Kupper MB, Rezende de Castro R, Bennink S, Glupczynski Y, Galleni M, Hoffmann KM, Bebrone C. Kinetic and crystallographic studies of extended-spectrum GES-11, GES-12, and GES-14
B-lactamases. Antimicrob Agents Chemother. 2012 Nov:56(11):5618.

Reset
Help
cephalothin
amoxicillin
aztreonam
ceftazidime
imipenem
cefotaxime
Best substrate (highest enzyme efficiency)
Worst substrate (lowest enzyme efficiency)
O The correct ranking cannot be determined.

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