Create a graph illustrating the activity of an enzyme or rate of reaction with the different factors affecting enzyme activity: 1) concentration of enzyme, 2) concentration of substrate, 3) temperature, 4) pH
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- 19. Define the terms “cofactor” and “coenzyme.” 20. What is the difference between general acid-base catalysis and specific acid-base catalysis? (Assume that the solvent is water.)22. In Exergonic reaction, all of the following are true except A. Spontaneous reaction B. Delta G is positive C. free energy of substrate is more than that of products D. catabolic reaction2c which is altered by a catalyst such as an enzyme, the net free energy change of the reaction or the activation energy of the reaction?
- 17. The prefix glycol comes from the Greek word that means “sweet” and the suffix lysis fromGreek word that means “to loosen” Explain how the meaning of these word parts arerelated to definition of glycolysis.12. Explain why those biological reactions that have their equilibria shifted towards theproducts have negative values for ΔGo of reactions. Explain how equilibria relates toGibbs free energy.Dilution factor problem in buffer and unbuffered solution in enzyme. In factors affecting enzyme activity (pH, temeprature). In testing pH factors, 2% of 4 mL of unbuffered starch solution was added to 4 mL of buffered starch solution, plus the 2mL enzyme solution. However, in testing temperature, 1% of 8 mL buffered starch solution was added to 2mL of enzyme solution. Why use different 1% and 2% starch concentration? My teacher said that in the pH part, upon adding the volumes, it will be diluted to 1%. i don't understand how he got it? If I use M1V1=M2V2, the answer is clearly not 1%. I am very confused. Thank you.
- 8. Anaplerotic and amphibolic reactions in TCA.22. Which of the following processes results to the formation of D-glucuronic acid in biological systems?A. Oxidation of the carbonyl carbon atom of glucoseB. Oxidation of the first chiral carbon atom of glucoseC. Oxidation of the penultimate carbon atom of glucoseD. Oxidation of the non-chiral alcoholic carbon of glucose23. Peptidoglycan is composed of two monosaccharide units. These are N-acetyl muramic acid and _____.A. β-L-fucoseB. N-acetyl-β-D-glucosamineC. N-acetyl-β-D-galactosamineD. Β-D-galactosamineKetone bodies are used as an alternative source of fuel during starvation. Describe how ketones are synthesized.
- Factors Affecting Enzyme Activity: Catalase 3. What is the marker for the enzyme’s optimum condition?1. Can the absorbance readings be used as a measure of enzyme activity atdifferent pH’s? 2. Describe an industrial application in which control of temperature and pHare critical to optimal enzymatic function.Glycolysis: Summary: Where does it occur?