Record pH of 0.1 M tris-HCI buffer after adding: PH meter 0 ml I ml 2 ml 3 ml 4 ml 5 ml 6 ml 7 ml Of: standardized between PH 7 &4 7.64 7.42 7.01 6.32 1.74 0.5 M HCL 7 & 10 7.92 8.13 8.33 8.53 8.71 10.9 0.5 M NaOH

Chemistry: Principles and Reactions
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Author:William L. Masterton, Cecile N. Hurley
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Chapter14: Equilibria In Acid-base Solutions
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Chemistry Buffering capacity of prepared buffers 3)
Record pH of 0.1 M tris-HCI buffer after adding:
PH meter
0 ml
I ml
2 ml
3 ml
4 ml
5 ml
6 ml
7 ml
Of:
standardized
between PH
7 &4
7.64
7.42
7.01
6.32
1.74
0.5 M HCL
7 & 10
7.92
8.13
8.33
8.53
8.71
10.9
0.5 M NaOH
Transcribed Image Text:Record pH of 0.1 M tris-HCI buffer after adding: PH meter 0 ml I ml 2 ml 3 ml 4 ml 5 ml 6 ml 7 ml Of: standardized between PH 7 &4 7.64 7.42 7.01 6.32 1.74 0.5 M HCL 7 & 10 7.92 8.13 8.33 8.53 8.71 10.9 0.5 M NaOH
3) Prepare 250 ml of 0.1 M Tris-HCI buffer, pH 7.8 as follows:
Weigh 3.029 g of Tris = Tris (hydroxymethyl) aminomethane, m.wt. 121.14
Add 2.78 ml of 6N HCI
pK of Tris = 8.1
SHOW CALCULATIONS IN YOUR LAB. REPORT
For the assay, measure 50 ml of each buffer into two 100 ml beakers. Use a clean
50 ml cylinder for each buffer.
Transcribed Image Text:3) Prepare 250 ml of 0.1 M Tris-HCI buffer, pH 7.8 as follows: Weigh 3.029 g of Tris = Tris (hydroxymethyl) aminomethane, m.wt. 121.14 Add 2.78 ml of 6N HCI pK of Tris = 8.1 SHOW CALCULATIONS IN YOUR LAB. REPORT For the assay, measure 50 ml of each buffer into two 100 ml beakers. Use a clean 50 ml cylinder for each buffer.
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