Q: All of the following statements about the repressor of the lac operon of E. coli are true EXCEPT…
A: The lac operon is a clustered group of related genes that are transcribed as a single unit. These…
Q: For each of the following transcription factors, explain how eukaryotic transcriptional initiation…
A: A transcription factor is a sequence specific DNA-binding factor and upon binding to the DNA…
Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…
A: Operon acts as a single regulated unit having one or more structural genes, promoter gene, a…
Q: In the trp operon, tryptophan itself serves as the effector for the trp repressor which causes it to…
A: trp operon is also known as Tryptophan operon An operon are gene clusters coding structural proteins…
Q: If you add a co-eepressor to a repressor protein and that allows it to bind the operator is this a…
A: Inducible operon is one that is expressed in the presence of inducer. Constitutive operon is the…
Q: The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose…
A: The prokaryotic gene regulation is performed by the operon system. In this system the polycystronic…
Q: What effect will the above process have on the tryptophan operon? RNA polymerase will detach from…
A: An operon is a functional unit of DNA (Deoxyribonucleic acid) containing a cluster of genes under…
Q: Which of the following statements about the trp operon is false?
A: From above question the statement If a mutation in region 2 prevents the formation of the 2 + 3…
Q: a mutation that leads to a nonfunctional adenylate cyclase enzyme would lead to what expression…
A: A mutation is a change in the genetic material (the genome) of a cell of a live creature or a virus…
Q: In response to potentially toxic substances (e.g., high levels of iron), eukaryotic cells often use…
A: Post-transcriptional modifications of pre-mRNA molecules like capping, splicing, and…
Q: If the two trp codons in the leader sequence were mutated to stop codons, what effect would it have…
A: DNA is the preferred carrier of genetic information in most living organisms except for certain RA…
Q: If a repressor prevents TFIID from binding to the TATA box, why does this inhibit transcription?
A: TFIID - It is an important transcription factor, which plays a key role in the activity of RNA…
Q: Which of the following is false when the genes of the Lac operon are being tran- scribed in high…
A: Lac operon is a set of genes used by E.coli to metabolize lactose when glucose is not available for…
Q: . Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of…
A: The bacterial transcription unit containing more than one gene is known as an operon. An operon has…
Q: n the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan is…
A: An operon is a functional unit of DNA (Deoxyribonucleic acid) containing a cluster of genes under…
Q: Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E.…
A: Since we only answer up to 3 sub-parts, we’ll answer the first 3. Please resubmit the question and…
Q: Which of the following results in transcriptional attenuation?
A: In hereditary qualities, attenuation is a proposed mechanism of control in some bacterial operons…
Q: The attenuation mechanism that helps block expression of the tryptophan operon requires all of the…
A: In transcriptional attenuation, the expression of genes are negatively regulated by controlling the…
Q: A bacterial cell is mutated and cannot create the repressor protein associated with the Lac operon.…
A: Lac operon is associated with uptake and metabolism of lactose. It has several components such as a…
Q: e following genetic and cellular conditions. You do not need to consider the role of CAP in your…
A: Upper operon - Is is super repressor. Once it is present in the sequence, it can not be removed. If…
Q: A mutation that inactivates transcription and translation from the regulatory gene of an inducible…
A: An operon is a functional unit of genomic DNA that comprises a collection of genes that are all…
Q: If there was a mutation such that the lac I protein could no longer bind lactose, but could still…
A: Lac operon is responsible for the metabolism of lactose in bacteria.
Q: What type of mutation in an operon is most likely to affect the synthesis of more than one protein?
A: Mutation is the process, in which genetic sequence is changed, which results in the change in…
Q: What effect will the above process have on the tryptophan operon? RNA polymerase will detach from…
A: Regulation of gene expression is the mechanism by which the genes are switched off and on depending…
Q: What is the function of cAMP in the regulation of the lac operon and how is the production of cAMP…
A: The Lac operon is a set of genes in prokaryotic cells that regulate lactose metabolism. An operator…
Q: For the following types of transcriptional control, indicate whether the protein produced by the…
A: An inducible operon is one which is not transcribed. For transcription of the molecule, it requires…
Q: A mutation prevents the catabolite activator protein (CAP) from binding to the promoter in the lac…
A: Mutation means sudden changes occur in DNA sequences. The mutation occurs randomly. It also occurs…
Q: The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a…
A:
Q: What is the major difference between the mechanism involved inattenuation and riboswitches and the…
A: Attenuation is a mechanism that controls gene expression in a bacterial operon and causes premature…
Q: What is the difference between positive regulation of the lac operon and negative regulation of the…
A: The lac operon is a set of three bacterial genes under the control of a single promoter which is…
Q: In the trp operon, tryptophan is best characterized as being a: A. repressor B. inducer C.…
A: Operon is clustered of Genes where a single mRNA consists of more than one functional gene.It is…
Q: In the lac operon, regulation of gene expression by CAP-CAMP is a type of O a. positive repressible…
A: Lac operon can be defined as the set of constitutive genes with a single promoter that encodes for…
Q: Which of the following mechanisms is an example of post-transcriptional gene regulation? A. binding…
A: The DNA (deoxyribonucleic acid) is the hereditary unit of an organism. It consists of purines and…
Q: Eukaryotic cells have multiple complex mechanisms for the regulation of gene expression, but a…
A: Answer :- a) Operons Explanation - Eukaryotic genes aren't organized into operons, therefore every…
Q: ac operon. Drag the listed molecules to their corresponding bin. Reset Help ONPG IPTG X-Gal…
A: ONPG - beta-galactosidase cleaves this molecule to form a visible yellow product. Explaination: The…
Q: If the above gene is one of the three structural genes of the lac operon that codes for the protein/…
A: Introduction Lactose operon(lac operon) is a system which includes genes that are essential for…
Q: When referring to attenuation in the regulation of the trp operon it would be safe to say that, when…
A: Attenuation is a regulatory mechanism that is employed in bacterial operons to guarantee that…
Q: For the following types of transcriptional control, indicate whether the protein produced by the…
A: Operon is a functional unit of DNA that consists of cluster of genes under control of promoter. Gene…
Q: For the ovalbumin gene shown, indicate the locations of the following: (a) transcription start site,…
A: The transcription start site is the site on the DNA from which the first RNA nucleotide (+1) is…
Q: Under which of the following conditions will transcription of the lac operon occur?a) Lactose…
A: Introduction All the gene expressions are under high regulation and this is necessary to avoid…
Q: In the tryptophan operon of E. coli, what happens when tryptophan levels get too high? tryptophan…
A: A trp operon is a group of genes that are transcribed, together to code for the components for the…
Q: For each of the following types of transcriptional control, indicate whether the protein produced by…
A: An inducible operon is one which is not transcribed. For transcription of the molecule, It requires…
Q: There is a mutation in sequence of the lac operator that prevents the lac repressor from being able…
A: Gene expression is regulated at the level of transcription by operon systems. In prokaryotes,…
Q: Which reaction normally happens in the regulation of the trp operon when high levels of tryptophan…
A: The trp repressor controls the trp operon. When coupled to tryptophan, the trp repressor prevents…
Q: What is the difference between the role of the operator and the role of the promoter in an operon
A: According to Jacob and monod concept, operon is a group of contiguous structural genes showing…
Q: A mutation at the operator prevents the regulator protein from binding.What effect will this…
A: Inducible operon has proteins that bind to repress or activate transcription. It depends on the…
Q: . Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of…
A: The bacterial transcription unit containing more than one gene is known as an operon. An operon has…
Q: When CAMP binds to the Catabolite Activator Protein (CAP), it causes a conformational change on the…
A: cAMP Cyclic Adenosine Monophosphate, it is an compound that act as secondary messenger and takes…
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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- Imagine that a new strain of E. coli is discovered, and it contains the following novel genetic region including the lacI gene and the lac operon: *see image* Upstream of the promoter associated with the lac operon, there is a new operator element "Oν" (O-"nu"). This new operator has high sequence homology to O1, the DNA element that is bound with the highest affinity by the lac repressor. What would be the impact of this new genetic element regarding behavior of the operonin the absence (vs. the presence) of lactose and allolactose? Answer in terms of the protein levels of the lac repressor, as well as the proteins encoded by the genes lacZ/Y/A in the downstream region of the operon. Remember that proteins, once expressed/generated, will not persist forever, and that they have a natural lifetime (ending when they are degraded by the cell).If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?Arginine is one of the amino acids that serves as a building block for protein synthesis. Arginine biosynthesis depends on the expression of the arg operon, which encodes ArgC (a reductase) and ArgB (acetylglutamate kinase). Which of the following statements regarding mutation of the arginine biosynthesis repressor ArgR is true? O A 1-bp insertion in the argR gene would result in a mutant able to grow in the absence of arginine, O A 1-bp deletion in the argB gene would result in a mutant unable to transcriptionally regulate the arg operon encoding argB. O A 1bp insertion in the argC gene would result in a mutant able to grow in the absence of arginine. O A 1-bp deletion in the argR gene would result in a mutant that represses the arg operon when grown in the presence of arginine. None of the above.
- You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence? Which repressor(s)under these conditions cannot bind the operator sequence? What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s…In addition to observing similarities to the lac operon, you also notice that this gene is regulated via attenuation, similar to the trp operon. Based on this similarity to this model operon, you could state that ___________. Group of answer choices If a terminator loop forms in the DNA, the expression of the structural genes is halted. The formation of the terminator hairpin followed by a series of Uracil (UUUUUUU) functions similar to Rho-Independent termination to result in the stopping of transcription. The transcription of a leader sequence affects the translation of the structural genes. Never mind – all of these statements are true! Attenuation will be the primary means of transcriptional regulation, with a repressor used as a back up option.Suppose you are studying the regulation of a gene involved in the metabolism of two nutrients, Llamasin and Alpacalon, in bacteria. You are trying to determine if these nutrients act as inducers in their operons. The following data were collected from your experiments. Which of these operons is most similar to the lac operon? Explain. Nutrient Levels of nutrient in growth medium Level of transcription of genes in operon Llamasin low high high low Alpacalon low low high high Highlight one in green: Llamasin Alpacalon Explanation:
- Describe the probable genetic defect that abolishes the sensitivity of the lac operon to the absence of glucose when other metabolic operons continue to be sensitive to the absence of glucose.You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, Please explain so I can understand in the answer What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s repressor?Consider the trp operon found in E. coli. You have discovered a mutant strain of E.coli that contains a mutation in its trpR gene. This mutation prevents the trpR protein from binding to its small effector molecule. What would happen if both the wild type and mutant bacteria were put in an environment that contained lots of tryptophan? The normal (wild type) bacteria would have (i)________expression of the trp operon while the mutant bacteria would have (ii) _________ expression of the trp operon. A) (i) high (ii) high B) (i) low (ii) low C) (i) high (ii) low D) (i) low (ii) high
- For the following questions, describe the expression levels of the structural genes in the Trp operon under the conditions described. In the absence of tryptophan, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of tryptophan, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the absence of tryptophan and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of tryptophan and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the absence of tryptophan and the trpR repressor gene is mutated such that the…For the following questions, describe the expression levels of the structural genes in the Trp operon under the conditions described. In the absence of tryptophan, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the presence of tryptophan, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"] In the absence of tryptophan and the promoter sequence was mutated such that the RNA polymerase can no longer bind, the structural genes will:[ Select ] ["No longer be expressed", "Be constantly expressed", "Be deleted from the operon"]What are the effects of the following conditions on Lac operon of bacteria? Please do not forget to mention about the role of repressor, activator, RNA polymerase in each case. Glucose is absent and lactose is present Glucose is present and lactose is present Glucose is present and lactose is absent