. Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E. coli. What will the most likely effect of each of these mutations be on the transcription of the trp structural genes? Q. A mutation that prevents the binding of the ribosome to the 5′ end of the mRNA 5′ UTR

. Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E. coli. What will the most likely effect of each of these mutations be on the transcription of the trp structural genes? Q. A mutation that prevents the binding of the ribosome to the 5′ end of the mRNA 5′ UTR

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 13TYU: INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following...

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. a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a singletranscript of the trp operon?b. How would you expect deletion of the two tryptophan codons in the RNA leader to affect theexpression of the trpE and trpC genes?
. Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E. coli. What will the most likely effect of each of these mutations be on the transcription of the trp structural genes? Q. Deletions in region 3 of the mRNA 5′ UTR
. Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E. coli. What will the most likely effect of each of these mutations be on the transcription of the trp structural genes? Q. A mutation that changes the Trp codons in region 1 of the mRNA 5′ UTR into codons for alanine
Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E. coli. What will the most likely effect of each of these mutations be on the transcription of the trp structural genes? a. A mutation that prevents the binding of the ribosome to the 5′ end of the mRNA 5′ UTR b. A mutation that changes the Trp codons in region 1 of the mRNA 5′ UTR into codons for alanine c. A mutation that creates a stop codon early in region 1 of the mRNA 5′ UTR d. Deletions in region 2 of the mRNA 5′ UTR e. Deletions in region 3 of the mRNA 5′ UTR f. Deletions in region 4 of the mRNA 5′ UTR g. Deletion of the string of adenine nucleotides that follows region 4 in the 5′ UTR
In addition to observing similarities to the lac operon, you also notice that this gene is regulated via attenuation, similar to the trp operon. Based on this similarity to this model operon, you could state that ___________. Group of answer choices If a terminator loop forms in the DNA, the expression of the structural genes is halted. The formation of the terminator hairpin followed by a series of Uracil (UUUUUUU) functions similar to Rho-Independent termination to result in the stopping of transcription. The transcription of a leader sequence affects the translation of the structural genes. Never mind – all of these statements are true! Attenuation will be the primary means of transcriptional regulation, with a repressor used as a back up option.
Bacterial DNA containing an operon encoding three enzymes is introduced into chromosomal DNA in yeast (a eukaryote) in such a way that it is properly flanked by a promoter and a transcriptional terminator. The bacterial DNA is transcribed and the RNA correctly processed, but only the protein nearest the promoter is produced. Can you suggest why?
If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
One of the main lessons of this chapter is that several bacterial genes are often transcribed from a single promoter into a large multigene (polycistronic) transcript. The region of DNA containing the set of genes that are cotranscribed, along with all of the regulatory elements that control the expression of these genes, is called an operon. a. Which of the mechanisms in the following list (i-vi) could explain differences in the levels of the mRNAs for different operons? Different promoters might have different DNA sequences Different promoters might be recognized by different types of RNA polymerase The secondary structures of mRNAs might differ which will influence the rate at which they are degraded by ribonucleases. In an operon, some genes are farther away from the promoter than other genes The translational initiation sequences at the beginning of different open reading frames in an operon might result in different efficiencies of translation Proteins encoded by different genes…
How will transcription of the E. coli trp (tryptophan) operon be affected by the following manipulations of the leader region of the trp mRNA?(a) Increasing the distance (number of bases) between the leader peptide gene and region 2(b) Increasing the distance between region 2 and region 3(c) Removing region 4(d) Changing several nucleotides in region 3 so that in can base pair with region 4 but not with region 2
1. a) If glucose is not available and lactose is absent from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon? b)If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon? b)
Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absent