Renata enzymatically conjugates a l"C-labeled cysteine to a transfer RNA (TRNA), with a UGU anticodon. Then, she chemically modifies the cysteine group to valine. Finally, she adds the altered aminoacyl-tRNA to a protein-synthesizing system containing normal components and a messenger RNA (MRNA) with the sequence 5'-UUUUGCCAUGUUUGUGCU-3'. What is the sequence of the radiolabeled peptide Renata produces? Enter your answer using three-letter abbreviations for each residue, separated by single hyphens. You may need to reference a codon table. Phe-Cys-His-Val-Ala-Ala radiolabeled peptide sequence: Incorrect

Renata enzymatically conjugates a l"C-labeled cysteine to a transfer RNA (TRNA), with a UGU anticodon. Then, she chemically modifies the cysteine group to valine. Finally, she adds the altered aminoacyl-tRNA to a protein-synthesizing system containing normal components and a messenger RNA (MRNA) with the sequence 5'-UUUUGCCAUGUUUGUGCU-3'. What is the sequence of the radiolabeled peptide Renata produces? Enter your answer using three-letter abbreviations for each residue, separated by single hyphens. You may need to reference a codon table. Phe-Cys-His-Val-Ala-Ala radiolabeled peptide sequence: Incorrect

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 20QP: If an extra nucleotide is inserted in the first exon of the beta globin gene, what effect will it...

See similar textbooks

Similar questions

A synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-Pro- Ile-Ser-Ala. What would be the effect on translation if the following components were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning. a. Initiation factor 3 b. Initiation factor 2 c. Elongation factor Tu d. Elongation factor G e. Release factors RF-1, RF-2, and RF-3 f. ATP g. GTP
What will be the overall anti-codon sequence in tRNA for this mRNA? 5’-GUAGCCUUAUCUAGCGAUCACCGUCCGUAUUACUAGUGGCCAGACUCUUUUCACCAUGUAUAGUUG-3’
Two missense mutations in the gene that encodes an enzyme called superoxide dismutase cause a form of amyotrophic lateral sclerosis (ALS, or Lou Gehrig disease). This disease causes loss of neurological function over a 5-year period. One mutation alters the amino acid asparagine (Asn) to lysine (Lys). The other changes an isoleucine (Ile) to a threonine (Thr). List the codons involved and describe how single-base mutations can alter the specified amino acids.
Consider the following tRNAs, where the numbered forms represent the amino acids associated with them, answer briefly: PICTURE Question 1: The numbering indicates the order in which these tRNAs are recruited to the A site of the ribosome. Write the sequence of the mRNA being translated in the 5' - 3' direction Question 2: What is the amino acid sequence of the produced polypeptide? Question 3: Researchers discover that a mutation is in the anticodon of the gene encoding the proline tRNA of an individual. The anticodon sequence is normally 3' GGA 5', but in this individual the anticodon sequence is 3' GGG 5'. It appears that this individual suffers no adverse consequences. How can this be? (2 response items)
A mutation is found in a tRNA-encoding gene. The wild type (non-mutant) allele (version) produces a tRNA that recognizes the codon GAA, and is charged with the amino acid glutamic acid (Glu). The mutant tRNA is still charged with Glu, but it recognizes the codon UAA. What effect will this have on translation in these cells? How will the proteins produced be different? Speculate: is this mutation more likely to be beneficial or harmful?
What is the peptide encoded by this mRNA sequence 5’-UCG-GCA- AAU-UUA -GUU-3’?
. Eukaryotic processing of hnRNA into mature mRNA includes all of the following steps except: ribosome attachment of methionine to the 5’-AUG-3’ codon 5’-addition of a 7-methylguanosine cap 3’-addition of a polyadenylated tail splicing together of exons excision of introns
If methionine is always the first amino acid incorporated into an oligopeptide, what oligopeptide is coded for by the following stretch of mRNA?
The mRNA formed from the repeating tetranucleotide UUACincorporates only three amino acids, but the use of UAUC incorporates four amino acids. Why?
Following is the sequence of a segment of mRNA: CGA AAA GUU UUU What are the anticodons of the tRNAs that will be used for translating this mRNA segment? Group of answer choices a. CGA, AAA, GUU, and UUU b. ARG, LYS, VAL, and PHE c. GCU, UUU, CAA, and AAA d. Arg-Lys-Val-Phe e. GCT, TTT, CAA, and AAA
A synthetic mRNA added to a cell-free protein-synthesizing system produces a peptide with the following amino acid sequence: Met-ProIle-Ser-Ala. What would be the effect on translation if the following component were omitted from the cell-free protein-synthesizing system? What, if any, type of protein would be produced? Explain your reasoning. Q. Initiation factor 3
Translate the following mRNA into protein, starting from the first initiation codon: 5'-CCGAUGCCAUGGCAGCUCGGUGUUAC AAGGCUUGCAUCAGUACCAGUUUGAAUCC-3'