13In growth medium containing glucose, lactose, and NR3003 an inhibitor of the enzyme creating cyclic adenosinemonophosphatethe expression of the lac operon is regulated in growth medium, the expression of the lac operon isrepressed, CAMP levels are high, the lac repressor is active, CAP is active, and RNA polymerase does not bind to the promoter.repressed, cAMP levels are, high, the lac repressor is active, CAP is inactive, and RNA polymerase does not bind to the promoter.repressed. CAMP levels are high, the lac repressor is inactive, CAP is inactive, and RNA polymerase does not bind to thepromoter.00expressed, cAMP levels are low, the lac repressor is active, CAP is active, and RNA polymerase bind to the promoterexpressed, CAMP levels are low, the lac repressor is active, CAP is inactive, and RNA polymerase bind to the promoter.expressed, cAMP levels are low, the lac repressor is inactive, CAP is active, and RNA polymerase bind to the promoter.All of the aboveNone of the above

Introduction: The lac operon is a genetic control element found in bacteria that regulates the…

Answered: rowth medium contain. ophosphate…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Maltose utilization in E. coli requires the proteins encoded by genes in three different operons. One operonincludes the genes malE, malF, and malG; the secondincludes malK and lamB; and the genes in the thirdoperon are malP and malQ. The MalT protein is apositive regulator that controls the expression of allthree operons; expression of the malT gene itself iscatabolite sensitive.a. What phenotype would you expect to result from aloss-of-function mutation in the malT gene?b. Do you expect the three maltose operons to containbinding sites for CRP (cAMP receptor protein)?Why or why not?In order to infect E. coli, bacteriophage λ binds to themaltose transport protein LamB (also known as the λreceptor protein) that is found in the outer membraneof the bacterial cell. The synthesis of LamB is induced by maltose in the medium via expression of theMalT protein, as described above.c. List the culture conditions under which wild-typeE. coli cells would be sensitive to infection bybacteriophage…
Assuming that the level of glucose is low, a mutation in the repressor associated with the lac operon of E. coli which prevents binding of the repressor to lactose should result in_____ Constitutive expression of the lac operon genes ito sagot ko Lack of expression or reduced expression of the lac operon genes under all circumstances Expression of the genes only when lactose is present Expression of the genes only when lactose is absent
The molecules indicated below can be used experimentally due to their roles in relation to the expression of the lac operon. Drag the listed molecules to their corresponding bin. Reset Help ONPG IPTG X-Gal allolactose Dragable beta-galactosidase cleaves this molecule to form a visible yellow product. beta-galactosidase cleaves this molecule which then dimerizes to form a visible blue product. This molecule induces the transcription of the lac operon by inactivating the lac repressor.
High levels of glucose in the environment result in low levels of transcription of the lac operon. The reason for this is ____________________________________________. glucose lowers the levels of the CAP protein, which is necessary to stimulate transcription of the lac operon glucose binds to the Lac Repressor to cause it to bind to the operator site (lac O) glucose raises the levels of cAMP, which binds to the CAP protein to cause transcription repression glucose prevents lactose from binding to the Lac Repressor glucose lowers the levels of cAMP, which is necessary to activate the CAP protein
For the lac operon, when glucose is absent and lactose is present ___________ None of the options cAMP-CAP is bound to the operator and the repressor is not bound to the promoter cAMP-CAP is not bound to the promoter and the repressor is bound to the operator cAMP-CAP is bound to the promoter and the repressor is not bound to the operator
consider the fec operon of Staphylococcus madeupiddis, which encodes an iron citrate uptake system. The fec operon promoter, called “Pfec”, is normally off when no regulators are bound to it (like the lac operon promoter). The activator FecA senses iron citrate and brings the appropriate enzyme to Pfec to start fec transcription when iron citrate levels are high. The repressor FecB senses free iron atoms and prevents fec transcription when free iron levels are high by binding to an operator between the promoter and structural genes. Considering the fecoperon described above, under which conditions would the operon be off? Choose all that apply. Low levels of free iron and high levels of citrate High levels of free iron and low levels of citrate High levels of free iron and high levels of citrate Low levels of free iron and low levels of citrate
consider the fec operon of Staphylococcus madeupiddis, which encodes an iron citrate uptake system. The fec operon promoter, called “Pfec”, is normally off when no regulators are bound to it (like the lac operon promoter). The activator FecA senses iron citrate and brings the appropriate enzyme to Pfec to start fec transcription when iron citrate levels are high. The repressor FecB senses free iron atoms and prevents fec transcription when free iron levels are high by binding to an operator between the promoter and structural genes. Considering the fec operon described above, which mutation would result in the operon being off under all conditions? Deletion of the operator where the repressor FecB binds to the DNA Deletion of the gene encoding the repressor FecB None of these mutations would result in the operon being off under all conditions Deletion of the gene encoding the activator FecA
Mutations in the genes of the lac operon might affect the regulation of β-galactosidase synthesis. For each mutation listed below, indicating whether β-galactosidase would be regulated normally, always ON or always OFF. Mutation in operator site prevents repressor binding Mutation in lacIgene prevents repressor from binding operator Mutation in lacIgene prevents repressor from binding allolactose Nonsense mutation in lacZgenere
Which of the following condition(s) is optimal for expression of the lac operon? I. low glucose and formation of CAP-cAMP complex II. high glucose resulting in no cAMP III. no lactose present and a functional repressor IV. presence of lactose binding to a functional repressor
What are the effects of the following conditions on Lac operon of bacteria? Do not forget to mention about the role of repressor, activator, RNA polymerase in each case! A) Glucose is absent and lactose is present B) Glucose is present and lactose is present C) Glucose is present and lactose is absent
The TRP operon is regulated by two mechanisms, the TrpR regulatory protein and attenuation. Complete following sentences by picking the right choice: 1. Attenuation occurs in the ___________ (promoter, structural genes, 5' UTR, region upstream of the promoter) , which is divided into four regions. It depends on the amount of ___________ (trp, tRNAs, charged trp tRNAs) in the cell, which is the signal for this type of regulation. 2. If the signal is low in the cell, then the ribosome pauses in region _______ (1, 2, 3, or 4) because the sequence includes several ______ (start codons, sotp codons, trp codons, trp molecules, inverted complementary sequences). Regions ________ (1 and 4, 1 and 3, 2 and 3, 2 and 4, 1 and 2, 3 and 4) will form a secondary structure and ______ (tranlsation continues, transcription stops, transcription continues, translation stops) (choose the first process impacted). 3. If the signal is high in the cell, then regions ______ (2 and 3, 2…
Select all of the cis-acting regulatory elements of the lac operon. Operator CAP+cAMP CAP+cAMP binding site LacZ

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rowth medium contain. ophosphate expression of the lac op