[S] (M) 2,5 x 10-6 Vo (μM/min) 28 4 x 10-6 40 1 X 10-5 70 2 x 10-5 95 4 x 10-5 112 1 x 104 128 2 x 10-3 139 1 x 10-2 140 2 x 10-1 140

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Chapter13: The Chemistry Of Solutes And Solutions
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Although there are accurate graphical methods available for determining Vmax and Km of an enzyme-catalyzed reaction, sometimes these parameters can be quickly estimated by examining the values of Vo as [S] increases. Estimate the values of Vmax and Km of the reaction catalyzed by an enzyme in which the following data were obtained:

[S] (M)
2,5 x 10-6
Vo (μM/min)
28
4 x 10-6
40
1 X 10-5
70
2 x 10-5
95
4 x 10-5
112
1 x 104
128
2 x 10-3
139
1 x 10-2
140
2 x 10-1
140
Transcribed Image Text:[S] (M) 2,5 x 10-6 Vo (μM/min) 28 4 x 10-6 40 1 X 10-5 70 2 x 10-5 95 4 x 10-5 112 1 x 104 128 2 x 10-3 139 1 x 10-2 140 2 x 10-1 140
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