Select all examples of DNA transformation Check All That Apply Transfer of DNA through a pilus into a bacterial cell in a laboratory. Transfer of DNA from media into a bacterial cell in a laboratory. Transfer of DNA through a pilus into a bacterial cell in seawater. Transfer of DNA from the environment into a bacterial cell via a surface receptor.

Select all examples of DNA transformation Check All That Apply Transfer of DNA through a pilus into a bacterial cell in a laboratory. Transfer of DNA from media into a bacterial cell in a laboratory. Transfer of DNA through a pilus into a bacterial cell in seawater. Transfer of DNA from the environment into a bacterial cell via a surface receptor.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 10QP: Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands...

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The restriction enzymes used in gene-cloning experiments, which generates sticky ends that can .a. cut the DNA, enter bacterial cellsb. cut the DNA, hydrogen bond with complementary sticky endsc. methylate DNA, enter bacterial cellsd. methylate DNA, hydrogen bond with complementarysticky ends
Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with ligaseb) Cleaving DNA with ligase and joining with endonucleasec) Cleaving and joining DNA with restriction endonucleased) Cleaving DNA with restriction endonuclease and joining with polymerase
Which of the following statements about DNA probes are false? a. Probes can be labeled with a fluorescent dye to facilitate detection. b. Probes can be labeled with radioactive phosphorus to facilitate detection. c. Probes are typically composed of double stranded pieces of DNA. d. Probes are typically composed of single stranded pieces of DNA.
Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction endonucleases primase dna polymerase resctriction exonuclease
Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
In order to insert foreign DNA into a plasmid for molecular cloning, the main components required include __________. (Check all that apply.) a selectable marker, usually antibiotic resistance a bacterial artificial chromosome one or more unique restriction sites an origin of replication
A genomic DNA library is made by using _____ to cut the target genome into small fragments, which are then amplified by PCR and ligated into plasmids.
Definition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified Organism
In your own words, explain how bacteria can be manipulated to contain a foreign DNA sequence or gene, how the expression of the gene could be monitored or selected for in the lab, and how the protein coded from this DNA sequence could potentially be purified. You must include at least 6 of the following terms in your answer: transformation, plasmid, antibiotic selection, fusion protein, chromatography, reporter gene, bioluminescence, competent cells, electroporation, calcium chloride.
. In this process, a gene of interest is inserted into the chromosome ofa bacterium, allowing the gene to be expressed within the cell.a. polymerase chain reactionb. DNA sequencingc. DNA cloningd. microarray technologye. DNA replication
Which of the following statements about gel electrophoresis is false? Choose all the answers that would apply -Smaller DNA fragments travel faster and further during gel electrophoresis. -DNA fragments can be separated based on fragment length. -DNA fragments can be separated based on DNA sequence. -Larger DNA fragments travel faster and further during gel electrophoresis.