Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Textbook Question
Chapter 13, Problem 10QP
Cloning Genes Is a Multistep Process
Which enzyme is responsible for covalently linking DNA strands together?
- a. DNA polymerase
- b. DNA ligase
- c. EcoRl
- d. restriction enzymes
- e. RNA polymerase
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Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- Which step follows the assembly of new DNA strands by DNA polymerase? A. Enzymes unwind and separate the two strands of DNA. B. Repair enzymes correct potential mutations in the DNA sequence. C. Primers base-pair with the exposed single DNA strands. D. DNA ligase seals any gaps remaining between bases of new DNA.arrow_forwardWhich step follows the assembly of new DNA strands by DNA polymerase? a.DNA ligase seals any gaps remaining between bases of new DNA. b.Primers base-pair with the exposed single DNA strands. c.Repair enzymes correct potential mutations in the DNA sequence. d.Enzymes unwind and separate the two strands of DNA.arrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forward
- Choose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptasearrow_forwardseparates molecules by movement due to size and electrical charge [ Choose ] a. DNA ligase b. gene cloning c. gel electrophoresis d. restriction enzymesarrow_forwarda. Describe the ingredients of a PCR reaction. b. List the different temperatures used in each cycle and tell why each is used.arrow_forward
- which process releases the DNA into the ionic medium? a. homogenization b. deproteination c. precipitation d. all are correctarrow_forwardWhy must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragmentarrow_forwardWhich helps prevent errors in DNA replication? A) Complementary base pairing reduce errors B) DNA ligase checks the DNA for errors C) DNA is located in the ribosomes D) Any base can pair with any other basearrow_forward
- Which is true of the lagging strand in DNA synthesis? a. It is built elongating towards the replication fork b. It is composed entirely of RNA c. It is built in the 3’ to 5’ direction d. It elongates in a series of segments, rather than continuouslyarrow_forwardWhich one is an example of nucleic acid hybridization? A the template and newly synthesized DNA strands in replication B reverse transcription of a human RNA into cDNA C association of two strands, one synthetic, by complementarity D synthesis of copy strands by PCR E gene cloning in bacteriaarrow_forward_____ can fix a genetic flaw in a human. a. Cloning b. Electrophoresis c. Sequencing d. Gene therapyarrow_forward
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