Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Textbook Question
Chapter 15, Problem 6QP
Which of the following best describes the process of DNA sequencing?
- a. DNA is separated on a gel, and the different bands are labeled with fluorescent
nucleotides and scanned with a laser. - b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments.
- c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR.
- d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser.
- e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
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Students have asked these similar questions
In next-generation sequencing, which of these advances allows for massively parallel sequencing?
a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece.
b. DNA sequences are read in real-time as nucleotides are added to each piece.
c. Each segment of the genome can be pieced back together through shotgun alignment
d. Single molecules of DNA can be read without the need for amplification.
DNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel?
a. The 2000-bp fragment
b. The 1000-bp fragment
c. The 500-bp fragment
d. All will migrate equal distances.
Arrange the following steps in the sequence they would happen in a DNA cloning experiment.
a. sealing DNA fragments into vectors with DNA ligase;
b. utilizing a probe to detect a clone in the library;
c. sequencing the clone's DNA;
d. creating a DNA library of clones;
e. cutting genomic DNA with restriction enzymes.
A.
e,a,d,b,c
B.
a,d,b,c,e
C.
c,b,e,a,d
D.
e,d,a,c,b
Chapter 15 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 15.1 - Who Owns Your Genome? John Moore, an engineer...Ch. 15.1 - Who Owns Your Genome? John Moore, an engineer...Ch. 15 - James sees an online ad for an at-home genetic...Ch. 15 - James sees an online ad for an at-home genetic...Ch. 15 - James sees an online ad for an at-home genetic...Ch. 15 - James sees an online ad for an at-home genetic...Ch. 15 - The gene controlling ABO blood type and the gene...Ch. 15 - Hemophilia and color blindness are both recessive...Ch. 15 - Prob. 3QPCh. 15 - Prob. 4QP
Ch. 15 - How many nucleotides does the human genome...Ch. 15 - Which of the following best describes the process...Ch. 15 - Which of the following is NOT an activity carried...Ch. 15 - Prob. 8QPCh. 15 - Prob. 9QPCh. 15 - What percentage of the DNA in the genome actually...Ch. 15 - When the human genome sequence was finally...Ch. 15 - One unexpected result of the sequencing of the...Ch. 15 - Prob. 13QPCh. 15 - Prob. 14QPCh. 15 - Prob. 15QPCh. 15 - Prob. 16QPCh. 15 - Prob. 17QP
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- Would it be possible to use human polymerase for the PCR reaction? a. No, because human polymerase does not have the ability to withstand the high temperatures required for the PCR reaction to occur. b. No, because human polymerase cannot be extracted from cells to use in a lab setting. c. Yes, because we are using human DNA as the template DNA. d. Yes, because human polymerase can add bases to a template strand without a primer.arrow_forwardWhich of the following is true? a. In one technique, the DNA sequence can be determined using one strand of DNA as it passes through a nanopore. b. Next generation sequencing is slow but accurate. c. Both of the above are true.arrow_forwardA contig is a. a set of molecular markers used in gene mapping. b. a set of overlapping fragments that form a continuous stretch of DNA. c. a set of fragments generated by a restriction enzyme. d. a small DNA fragment used in sequencing.arrow_forward
- Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardPut the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardFrom where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forward
- What are the main components needed for electrophoresis? a) A power source b) A gel matrix c) A medium (or "buffer") d) Casting tray e) All of the above For what purpose is DNA electrophoresis used? a) To amplify a DNA for cloning b) To separate noncharged molecules of DNA based on size c) To separate charged molecules of DNA based on size d) To separate charged molecules of DNA based on amount of positive charge e) To produce static electricity by frictionarrow_forwardPCR (polymerase chain reaction) is an excellent method of generating copies of target DNA. If a single piece of double stranded DNA (dsDNA) is put into a PCR machine, how many dsDNA segments will there be after 3 rounds? A. 8 segments, with 2 original strands paired B. 16 segments, with 2 original strands on different segments C. 16 segments, with 2 original strands paired D. 8 segments, with 2 original strands on different segmentsarrow_forwardBased on what you already know, explain two reasons why is it important for scientists to beable to remove DNA from an organism? Keep in mind that an organism includes any livingthing, from bacteria to humans.arrow_forward
- Polymerase Chain Reaction, or PCR, can Group of answer choices A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis. B. copy the number of copies of a selected region of DNA linearly. C. increase the number of copies of a selected region of DNA exponentially. D. copy the entire genome at least a dozen times.arrow_forwardChoose the one answer that fits best. Which statement regarding PCR is NOT correct (videos)? a. PCR requires a copy of RNA that serves as a template b. Taq polymerase adds nucleotides to the primers and creates a complementary strand of DNA c. Annealing requires cooler temperatures than denaturation d. Repeated cycles of denaturation, annealing and extending DNA strands creates many identical copies of DNA e. PCR is a quick way of using minute quantities of DNA to create millions of copiesarrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, how many cuts were performed on DNA sequence A? A.) 2 B.) 3 C.) 4arrow_forward
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