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Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.

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Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052

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BuyFindarrow_forward

Human Heredity: Principles and Iss...

11th Edition
Michael Cummings
Publisher: Cengage Learning
ISBN: 9781305251052
Chapter 15, Problem 6QP
Textbook Problem
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Which of the following best describes the process of DNA sequencing?

  1. a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser.
  2. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments.
  3. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR.
  4. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser.
  5. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.

Summary Introduction

Introduction: DNA sequencing method was developed in the 1970s. It is a method of sequencing DNA to determine the position of nucleotides in DNA. Sanger method is one of the techniques used to determine the location of nucleotides in DNA.

Explanation of Solution

Reason for correct answer:

Sanger method uses four different fluorescent dyes, one for each base-A, T, G, and C. The fragments of DNA produced in the chemical reactions and they are separated in four adjacent lanes in a gel. This method is useful for sequencing small amounts of DNA.

Option d. is given as “Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then run on a gel and scanned with a laser.”

Sanger method uses four separate chemical reactions with fluorescent nucleotides (fluorescent dyes). The DNA fragment produced by chemical reactions is then run on a gel and scanned with a laser. Hence, the correct answer is option d.

Reasons for the incorrect answer:

Option a. is given as, “DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser.”

DNA sequencing can be done by Sanger method and High Throughput sequencing techniques or Next-Generation sequencing methods. The Sanger method uses four chemical reactions to separate DNA fragments, and then these DNA fragments run on a gel. Hence, option a. is incorrect...

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