Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.

Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.10: The Microscope

Problem 18RQ

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Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
Which of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope? Question options: Contrast Resolving power All of the choices Numerical aperture
Which of these is NOT a best practice when using, handling, or storing a microscope and microscope slides? Do not touch the objective lenses. Store the slides in bright sunlight to prevent mold growth. Rotate the nosepiece to the lowest objective before storing the microscope. Keep dust away from the scope by covering it during storage.
Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the center
How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity
As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images. a. refractive index b. light absorption c. natural fluorescence d. walls and coverings e. natural pigmentation
Can someone answer and explain the following? What are the basic components of a Fluorescence Microscope and what are the functions of each? Are there any parts that you can remove without compromising accuracy and utility of the equipment? Can you suggest additional components to improve the equipment?
Write the term that the phrase describes. 1. Large knob that moves the stage or objective lens a great distance. Used with scanning or low-power objective lenses only. 2. Flat platform beneath the objective lens on which the microscope slide is placed. 3. Removable lenses that you look through to observe the microscope slide. 4. Small knob that moves the stage or objective lens a very small distance and is used for precision focusing. 5. Extends from the arm and contains the ocular lenses and rotating nosepiece. 6. Lens that condenses light through the specimen and is located below the stage. 7. Light from specimen passes through these lenses first. These lenses are located in the rotating nosepiece. 8. Wide bottom part that supports the microscope. 9. Regulates the amount of light passing through the condenser. 10. Vertical portion that connects the base to the head.
What are the advantages of using a Refractometer instead of other Specific Gravity measurement equipment used in Clinical Microscopy?
A student using a microscope measured the low power field diameter to be 7.1mm. The student then switched from the low power (4x) objective lens to the medium-power (10x) objective lens. Assuming a 10 x ocular (eyepiece) lens, the medium power field diameter is ____ um um is micrometers
List down 5 steps in the given procedure below for the proper use of microscope that you think emphasized on proper equipment care and briefly explain why you think so in 1-2 sentences per identified step. 1. Connect the microscope to the power supply. Turn “ON” the microscope.2. Rotate the light intensity adjustment knob to adjust the brightness.3. Place the slide with the specimen facing upwards on top of the mechanical stage. a. Open the bow-shaped lever of the stage clip outward.b. Slide the specimen from the front toward the rear.c. Return the bow-shaped lever gently.d. Center the specimen over the aperture on the stage. 4. Use the Low Power Objective. a. Rotate the revolving nosepiece until the 10x objective lens is “clicked” into position.b. Rotate the condenser focus knob to bring the condenser down to the bottom and partially open the iris diaphragm.c. Rotate the coarse adjustment knob to focus the image. Move it as far as it will go without touching the slide.d. When coarse…
True or false : each objective of a phase contrast microscope contains a phase plate. phase contrast microscopy relies on the condenser to increase the contrast of an image. in brightfield microscopy, closing the condenser aperture will decrease the contrast of the speciment. stained cell speciments are best viewed by phase contrast microscopy.