Need help with question 2:Which one is the more polar component? Explain. 1. A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase thancomponent B. The following result is obtained. Calculate the Rf for each component and label the identity of eachspot. 2Solvent Front00Spotting Line2. Which one is the more polar component? Explain3. If a more polar solvent (mobil phase) is used for the above experiment, the result will be:a) Both component A and Component B will travel further up the plateb) Only Component A will travel further up the platc) Only Component B will travel further up the platd) They will travel on the plate the same distance as when a less polarChoose one of the answers and explain4. Paper chromatography works as the same principle as TLC however, cellulose paper (the same as filter paper) isused for stationary phase. An unknown liquid sample is analyzed using paper chromatography using solvent X as themobile phase. One spot is observed after the plate is developed and visualized. The same unknown substance is re-analyzed using solvent Y as the mobile phase. This time, three spots are observed after the plate is developed andvisualized. Is the unknown sample a pure substance or a mixture? Explain your answer, including a possible reasonfor the different observations in the two experiments5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on a plate but it ispacked into a glass (or plastic column) and the eluting solvents runs down a column by gravitational pull.The mixture from question 1 (above) is analyzed by column chromatography using the same mobile phase and asilica gel stationary phase. Which component has the shorter retention time? Explain your answer. (Assume thesame type of binding forces between the components and the stationary phase are present in both paperchromatography and column chromatography.)

The compounds or molecules having a permanent dipole moment are polar in nature. Polar compounds are…

Answered: Solvent Front 0 H Spotting Line 2.…

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Solvent Front 0 H Spotting Line 2. Which one is the more polar component? Explain

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter28: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 28.19QAP

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The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67
a) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids shown below using silica gel as the stationary phase and ethyl acetate/hexanes as the mobile phase? Whichsolid would have the highest Rf and which would have the lowest Rf? Explain b) Would changing the mobile phase (from part a) and increasing thepolarity by introducing small amount of methanol to the mobile phase change the order ofRf of the above 3 compounds? Explain c) Another type of chromatography is Reverse Phase Chromatography. In this type ofchromatography, the stationary phase is non-polar and the mobile phase is polar. If astudent performed Reverse Phase TLC on the mixture of 3 solids (from part a),what would be the order of Rf’s of the compounds? Which would have the lowest Rf andwhich would have the highest Rf?
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