separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high- performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.
separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high- performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material. i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above. ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied. iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.
Chapter91: Gas Chromatography
Section: Chapter Questions
Problem 1P
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separate a mixture containing acetone, acetamide and 1,2-dichloroethane using a high- performance liquid chromatography (HPLC) technique. The instrument was equipped with a 40-cm column packed with cyano propyl bonded to the siloxane backbone material.
i) List THREE (3) examples of mobile phase solvent/solvent systems that can be used with the column above.
ii) Determine the sequence in which the three analytes eluted from the column and explain the underlying principle applied.
iii) Sketch the chromatogram that would be produced if the column was replaced with a column filled with C18 hydrocarbon bound to the siloxane backbone material and acetonitrile was employed as the mobile phase. Label the peaks observed.
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