Some of the electron transport carriers (the cytochromes) absorb light at characteristicwavelengths when they are carrying the extra electron from NADH but not when they give upthe electrons to the next carrier. Thus, light absorption bands are all present when mitochondriaare incubated in the absence of oxygen or when tyanide is present. Cyanide blocks the transferof electrons from the last cytochrome to oxygen. When cyanide is absent and oxygen isintroduced, the cytochromes stop absorbing light. There are three cytochromes in animalmitochondria, called a, b, and c. Another inhibitor of mitochor rial electron transfer is urethane.When urethane is added to aerobic mitochondria, cytochrome b begins to absorb light, but a andc do not. Finally, purified cytochrome c, even in the presence of oxygen, continues to absorblight. Use these observations to decide the order in which the cytochromes occur in themitochondria.

Answered: Some of the electron transport carriers…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter21: Photosynthesis

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Problem 5P: The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If...

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Some of the electron transport carriers (the cytochromes) absorb light at characteristic
wavelengths when they are carrying the extra electron from NADH but not when they give up
the electrons to the next carrier. Thus, light absorption bands are all present when mitochondria
are incubated in the absence of oxygen or when tyanide is present. Cyanide blocks the transfer
of electrons from the last cytochrome to oxygen. When cyanide is absent and oxygen is
introduced, the cytochromes stop absorbing light. There are three cytochromes in animal
mitochondria, called a, b, and c. Another inhibitor of mitochor rial electron transfer is urethane.
When urethane is added to aerobic mitochondria, cytochrome b begins to absorb light, but a and
c do not. Finally, purified cytochrome c, even in the presence of oxygen, continues to absorb
light. Use these observations to decide the order in which the cytochromes occur in the
mitochondria.

Expert Solution

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Introduction

The electron transport chain (ETC) is a series of protein complexes and electron carriers located in the inner mitochondrial membrane of eukaryotic cells or in the plasma membrane of prokaryotes. The ETC is a key component of aerobic respiration, which is the process by which cells convert nutrients into energy in the presence of oxygen.

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