Standard solutions of Erythrosin B (A red food dye) are prepared by pipetting 1.00, 2.00, 5.00, 7.00, 10.00 mL of 1.108x10- 2 mol/L Erythrosin B solution into 50.00 mL volumetric flasks. The 5 standards are then run on a Spectronic 20 at Amax Complete the table below and graph the calibration curve. [Erythrosin B] (mol/L) Volume Erythrosin B (mL) 1.00 2.00 5.00 7.00 10.00 Equation of trendline: R². Absorbance 0.160 0.211 0.342 0.450 0.571
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- A pipet is used to transfer 6.00 mLmL of a 3.75 MM stock solution in flask “S” to a 25.00-mL volumetric flask “A,” which is then diluted with DI H2OH2O to the calibration mark. The solution is thoroughly mixed. Next, 10.00 mLmL of the solution in volumetric flask “A” is transferred by pipet to a 50.00-mL volumetric flask “B” and then diluted with DI H2OH2O to the calibration mark. Calculate the molarity of the solution in volumetric flask “B.”The concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…Chemistry The levels of an organic pollutant (P) in the groundwater at the perimeter of a plant were a cause for concern. A 10 mL sample of the water was taken and the pollutant was extracted with 95% efficiency using 25 mL of diethyl ether. GC was used to analyse the concentration of P in diethyl ether. A calibration curve was plotted for a series of standards of P which yielded the following results: Peak Area Toluene Conc. (µg/ml) 12,000 2.6 23,700 5.0 35,500 7.7 46,800 9.9 31,250 Sample Determine the concentration of P in ppb in the initial groundwater sample.
- 125.1 mg of streptomycin sulphate are dissolved in 10 ml of water. A GC headspace analysis is carried out in order to determine the methanol content of the drug. A peak for methanol is produced which has 73.2% of the area of a peak for a methanol standard containing 0.532 mg/100 ml of methanol in water analysed under exactly the same conditions, What is the methanol content of the streptomycin sulphate in ppm and %w/w? Answer: 311.3 ppm, 0.3113 % w/w.how??4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in b1. What is the MW of the analyte?2. What is the meq of the analyte?3. What will be the weight (in grams) of the analyte if the standardization is to be quartered?4. What will be Normality of the solution if 7.5ml of the titrant was consumed in the standardization?5. What is the average Normality if two or more trials were conducted with the values of 0.102N and 0.105N?
- A second spectrophotometric method for the quantitative analysis of Pb2+ in blood yields an Ssamp of 0.712 for a 5.00 mL sample of blood. After spiking the blood sample with 5.00 μL of a 1560 ppb Pb2+ external standard, an Sspike of 1.546 is measured. What is the concentration of Pb2+ in the original sample of blood?The standard addition method is used to analyze a sample of a river water for mercury. Solution A is made by pipetting 5.00 mL of undiluted sample in to a 10 mL volumetric flask and filling to the mark with DI water. Solution B is made by pipetting 5.00 mL of undiluted sample and 3.00 mL of 15.0 ppb of Hg standard into same 10.0 mL volumetric flask and filling to the mark with DI. Solution A and B are analyzed using atomic absorption spectroscopy and give a percent transmittance values of 56 % and 33 % respectively (not blank corrected). A blank has a transmittance of 96%. What is the corrected absorbance of both solution A and B? A. Solution A: 0.123 Solution B: 0.463 B. Solution A: 0.463 Solution B: 0.234 C. Solution A: 0.123 Solution B: 0.234 D. Solution A: 0.234 Solution B: 0.463Six 50.0 ml volumetric flask 1-6 are labeled. 10.00 ml of 2.00 x 101 M Fe(NO3)3 solution was then pipet into each volumetric flask. A 1.00,2.00,3.00,4.00 and 5.00ml of 2.00 x 10-3M NaSCN solution was then pipetted to flask 2 through 6 respectively. A sufficient 0.10 M nitric acid was then added to each flask to make them total volume of 50.00ml. 1.a)Calculate the final Fe(NO3)3 concentrations for each. 1.b)Also how would you find the initial concentration of SCN- for each flask? Please show calculations to solutions.Thanks
- 1. What is the MW of the analyte?2. What is the meq of the analyte?3. What is the weight (in grams) of the analyte?4. What will be the percentage purity of the sample if 43mL of the titrant was consumed?5. Did the sample conform to the USP requirement? Yes or No?A stock solution of potassium permanganate (KMn04) was prepared by dissolving 13.0g KMn04 with DI in a 100ml volumetric flask and diluting to the calibration mark. Determine the molarity of the solution.Six 50.0 ml volumetric flask 1 through 6 are labeled. 10.00 ml of 2.00 x 10-1 M Fe(NO3)3 solution was then pipet into each volumetric flask. A 1.00,2.00,3.00,4.00 and 5.00ml of 2.00 x 10-3M NaSCN solution was then pipetted to flask 2 through 6 respectively. A sufficient amount of 0.10 M nitric acid was then added to each flask to make each a total volume of 50.00ml. Hint M1V1=M2V2 a)what would be the concentration of Fe(NO3)3 in 0.10M HNO3? b)what would be the concentration of NaSCN in 0.10M HNO3?